Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vasseur, M.; Frangne, R.; Cauzac, M.; Mahmood, A.; Alvarado, F.
    pH-Dependent inhibitory effects of tris and lithium ion on intestinal brush-border sucrase (1990), J. Enzyme Inhib., 4, 15-26.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.48 2-amino-2-methyl-1-propanol causes activation at pH 5.2 Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.48 Li+ high concentration, 300 mM Li+, pH dependent inhibition, complete at pH 8 Oryctolagus cuniculus
3.2.1.48 Tris concentration-dependent biphasic effect, first causing activation, fully competitive inhibition above pH 6.8, inhibition is stronger at alkaline pH values Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.48 15.9
-
 sucrose pH 7.6 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.48 maltose + H2O Oryctolagus cuniculus
-
 2 glucose
-
 ?
3.2.1.48 sucrose + H2O Oryctolagus cuniculus
-
 D-glucose + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.48 Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.48
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
3.2.1.48 4°C or -20°C, 0.02% sodium azide, Triton X-100, several weeks, sucrase-isomaltase Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.48 maltose + H2O
-
 Oryctolagus cuniculus 2 glucose
-
 ?
3.2.1.48 sucrose + H2O
-
 Oryctolagus cuniculus D-glucose + D-fructose
-
 ?