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Literature summary extracted from

  • Ohashi, T.; Hong, C.M.; Weiler, S.; Tomich, J.M.; Aerts, J.M.; Tager, J.M.; Barranger, J.A.
    Characterization of human glucocerebrosidase from different mutant alleles (1991), J. Biol. Chem., 266, 3661-3667.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.45 phosphatidylserine
-
 Homo sapiens
3.2.1.45 saposin-C sphingolipid activator protein, SAP-2 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.45 expression in NIH 3T3 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.45 L444P Gaucher disease mutation, reduced amounts of protein in cells Homo sapiens
3.2.1.45 N370S abnormal catalytic site Homo sapiens
3.2.1.45 P415R Gaucher disease mutation, reduced amounts of protein in cells Homo sapiens
3.2.1.45 R120Q Gaucher disease type I mutation, inactive Homo sapiens
3.2.1.45 R463C Gaucher disease type I mutation, less active with saposin C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.45 (1R,2R,3S,4S,5R,6S)-7-oxabicyclo[4.1.0]heptane-2,3,4,5-tetrol
-
 Homo sapiens
3.2.1.45 glucosylsphingosine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.45 2.7
-
 4-methylumbelliferyl-beta-D-glucopyranoside
-
 Homo sapiens
3.2.1.45 4
-
 4-methylumbelliferyl-beta-D-glucopyranoside recombinant and mutant enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.45 glucocerebroside + H2O Homo sapiens
-
 glucose + ceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.45 Homo sapiens
-
 normal and Gaucher disease enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.45 of the recombinant protein Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.45 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.45 4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens methylumbelliferone + glucose
-
 ?
3.2.1.45 glucocerebroside + H2O
-
 Homo sapiens glucose + ceramide
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.45 50
-
 60 min, no loss of activity Homo sapiens