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Literature summary extracted from

  • Liedtke, H.; Legler, G.
    Spleenic glucocerebrosidase and its cytosolic activator protein: effects on substrate hydrolysis and covalent inhibition by conduritol B epoxides (1988), NATO ASI Ser. A, Life Sci., 150, 353-358.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.45 Activator protein
-
 Bos taurus
3.2.1.45 Dicetyl phosphate
-
 Bos taurus
3.2.1.45 sodium taurocholate
-
 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.45 (1R,2R,3S,4S,5R,6S)-7-oxabicyclo[4.1.0]heptane-2,3,4,5-tetrol
-
 Bos taurus
3.2.1.45 6-bromo-6-deoxy-conduritol
-
 Bos taurus
3.2.1.45 6-bromo-conduritol-beta-epoxide
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.45 59000
-
-
 Bos taurus
3.2.1.45 64000
-
 gel filtration, SDS-PAGE Bos taurus
3.2.1.45 67000
-
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.45 glucocerebroside + H2O Bos taurus
-
 glucose + ceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.45 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.45
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.45 spleen
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.45 glucocerebroside + H2O
-
 Bos taurus glucose + ceramide
-
 ?
3.2.1.45 nonylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Bos taurus nonylumbelliferone + glucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.45 4.8 5.8 taurocholate shifts pH-optimum for hydrolysis of nonylumbelliferyl-beta-D-glucose from 5.8 to 4.9, activator protein shifts pH-optimum for hydrolysis of methylumbelliferyl-beta-D-glucose from 5.8 to 4.3 Bos taurus