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Literature summary extracted from

  • Kimura, T.; Horikoshi, K.
    Characterization of pullulan-hydrolysing enzyme from an alkalopsychotropic Micrococcus sp. (1990), Appl. Microbiol. Biotechnol., 34, 52-56.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Co2+
-
 Micrococcus sp.
3.2.1.41 Cu2+
-
 Micrococcus sp.
3.2.1.41 Cyclodextrins
-
 Micrococcus sp.
3.2.1.41 EDTA
-
 Micrococcus sp.
3.2.1.41 Hg2+
-
 Micrococcus sp.
3.2.1.41 Mn2+
-
 Micrococcus sp.
3.2.1.41 N-bromosuccinimide at 5 mM Micrococcus sp.
3.2.1.41 Ni2+
-
 Micrococcus sp.
3.2.1.41 Pb2+
-
 Micrococcus sp.
3.2.1.41 Sn2+
-
 Micrococcus sp.
3.2.1.41 Sodium dodecyl sulfate
-
 Micrococcus sp.
3.2.1.41 Zn2+
-
 Micrococcus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 extracellular
-
 Micrococcus sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+ enhances stability Micrococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 120000
-
 SDS-PAGE Micrococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Micrococcus sp. branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Micrococcus sp.
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Micrococcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 to homogeneity Micrococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Micrococcus sp. maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Micrococcus sp. maltooligosaccharides + maltose
-
 ?
3.2.1.41 glycogen + H2O
-
 Micrococcus sp. maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Micrococcus sp. maltotriose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type I hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan Micrococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 50
-
-
 Micrococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 40 55
-
 Micrococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 8
-
-
 Micrococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 7 9
-
 Micrococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.41 5 10 10% loss of activity in the presence of CaCl2 Micrococcus sp.