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Literature summary extracted from

  • Bertoldo, C.; Duffner, F.; Jorgensen, P.L.; Antranikian, G.
    Pullulanase type I from Fervidobacterium pennavorans Ven5: cloning, sequencing, and expression of the gene and biochemical characterization of the recombinant enzyme (1999), Appl. Environ. Microbiol., 65, 2084-2091.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.41 2-mercaptoethanol
-
 Fervidobacterium pennivorans
3.2.1.41 dithiothreitol
-
 Fervidobacterium pennivorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli Fervidobacterium pennivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Cu2+ at 5 mM Fervidobacterium pennivorans
3.2.1.41 Cyclodextrins
-
 Fervidobacterium pennivorans
3.2.1.41 Fe2+ at 5 mM Fervidobacterium pennivorans
3.2.1.41 Guanidine HCl
-
 Fervidobacterium pennivorans
3.2.1.41 iodoacetamide
-
 Fervidobacterium pennivorans
3.2.1.41 N-bromosuccinimide
-
 Fervidobacterium pennivorans
3.2.1.41 Ni2+ at 5 mM Fervidobacterium pennivorans
3.2.1.41 Sodium dodecyl sulfate
-
 Fervidobacterium pennivorans
3.2.1.41 Urea
-
 Fervidobacterium pennivorans
3.2.1.41 Zn2+ at 5 mM Fervidobacterium pennivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Fervidobacterium pennivorans branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O Fervidobacterium pennivorans Ven5 branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Fervidobacterium pennivorans
-
 maltotriose + ?
-
 ?
3.2.1.41 pullulan + H2O Fervidobacterium pennivorans Ven5
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Fervidobacterium pennivorans
-
 Ven5
-
3.2.1.41 Fervidobacterium pennivorans Ven5
-
 Ven5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 of the recombinant protein Fervidobacterium pennivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O
-
 Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
3.2.1.41 dextrin + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 glycogen + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 glycogen + H2O
-
 Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Fervidobacterium pennivorans maltotriose + ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Fervidobacterium pennivorans Ven5 maltotriose + ?
-
 ?
3.2.1.41 soluble starch
-
 Fervidobacterium pennivorans maltotriose + maltose
-
 ?
3.2.1.41 soluble starch
-
 Fervidobacterium pennivorans Ven5 maltotriose + maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type I hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan Fervidobacterium pennivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 80
-
-
 Fervidobacterium pennivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 80
-
 pH 6, after 2 h, 50% loss of activity Fervidobacterium pennivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Fervidobacterium pennivorans