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Literature summary extracted from

  • Kim, C.H.; Choi, H.I.; Lee, D.S.
    Purification and biochemical properties of an alkaline pullulanase from alkalophilic Bacillus sp. S-1 (1993), Biosci. Biotechnol. Biochem., 57, 1632-1637.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Cu2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.41 Fe2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.41 Fe3+
-
 Bacillus sp. (in: Bacteria)
3.2.1.41 Zn2+
-
 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+
-
 Bacillus sp. (in: Bacteria)
3.2.1.41 Co2+ slightly stimulated Bacillus sp. (in: Bacteria)
3.2.1.41 K+ slightly stimulated Bacillus sp. (in: Bacteria)
3.2.1.41 Mg2+ slightly stimulated Bacillus sp. (in: Bacteria)
3.2.1.41 Mn2+ highly stimulated Bacillus sp. (in: Bacteria)
3.2.1.41 Na+ slightly stimulated Bacillus sp. (in: Bacteria)
3.2.1.41 Ni2+ slightly stimulated Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 120000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Bacillus sp. (in: Bacteria) branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Bacillus sp. (in: Bacteria)
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose
-
 ?
3.2.1.41 dextrin + H2O
-
 Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose
-
 ?
3.2.1.41 glycogen + H2O
-
 Bacillus sp. (in: Bacteria) maltooligosaccharides + maltose
-
 ?
3.2.1.41 glycogen beta-limit dextrin + H2O
-
 Bacillus sp. (in: Bacteria) maltotriose + maltose + maltosetetraose
-
 ?
3.2.1.41 pullulan + H2O
-
 Bacillus sp. (in: Bacteria) maltotriose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type I hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 85
-
 stability increased by pullulan Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 8.5 9
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.41 4 11 stable at 4°C for 24 h Bacillus sp. (in: Bacteria)