Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Plant, A.R.; Clemens, R.M.; Daniel, R.M.; Morgan, H.W.
    Purification and preliminary characterization of an extracellular pullulanase from Thermoanaerobium Tok6-B1 (1987), Appl. Microbiol. Biotechnol., 26, 427-433.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 extracellular
-
 Thermoanaerobium sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+
-
 Thermoanaerobium sp.
3.2.1.41 Sr2+
-
 Thermoanaerobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 120000
-
 gel filtration Thermoanaerobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Thermoanaerobium sp. branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Thermoanaerobium sp.
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Thermoanaerobium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Thermoanaerobium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.41 1352 1790
-
 Thermoanaerobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Thermoanaerobium sp. maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Thermoanaerobium sp. maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylose + H2O
-
 Thermoanaerobium sp. maltooligosaccharides + maltose
-
 ?
3.2.1.41 maltotetraose + H2O slowly Thermoanaerobium sp. maltose + glucose + maltotriose
-
 ?
3.2.1.41 pullulan + H2O
-
 Thermoanaerobium sp. maltotriose + ?
-
 ?
3.2.1.41 soluble starch
-
 Thermoanaerobium sp. maltotriose + maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Thermoanaerobium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 80
-
 instable above Thermoanaerobium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.41 4830
-
 pullulan
-
 Thermoanaerobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 5.5
-
-
 Thermoanaerobium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 4 7.5
-
 Thermoanaerobium sp.