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Literature summary extracted from

  • Kusano, S.; Nagahata, N.; Takahashi, S.; Fujimoto, D.; Sakano, Y.
    Purification and properties of Bacillus acidopullulyticus pullulanase (1988), Agric. Biol. Chem., 52, 2293-2298.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 2.5
-
 pullulan glucose equivalents Bacillus acidopullulyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 115000
-
 1 * 115000, gel electrophoresis Bacillus acidopullulyticus
3.2.1.41 115000 116000 gel electrophoresis Bacillus acidopullulyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Bacillus acidopullulyticus branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Bacillus acidopullulyticus
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Bacillus acidopullulyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Bacillus acidopullulyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.41 commercial preparation promozym 200L Bacillus acidopullulyticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.41 200 220
-
 Bacillus acidopullulyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Bacillus acidopullulyticus maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Bacillus acidopullulyticus maltooligosaccharides + maltose
-
 ?
3.2.1.41 glycogen beta-limit dextrin + H2O
-
 Bacillus acidopullulyticus maltotriose + maltose + maltosetetraose
-
 ?
3.2.1.41 pullulan + H2O
-
 Bacillus acidopullulyticus maltotriose + ?
-
 ?
3.2.1.41 soluble starch
-
 Bacillus acidopullulyticus maltotriose + maltose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 monomer 1 * 115000, gel electrophoresis Bacillus acidopullulyticus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type I hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan Bacillus acidopullulyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 65
-
-
 Bacillus acidopullulyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 75
-
 not active above, pullulan Bacillus acidopullulyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 60
-
 instable above Bacillus acidopullulyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 5
-
-
 Bacillus acidopullulyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 3 7
-
 Bacillus acidopullulyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.41 4 8.5
-
 Bacillus acidopullulyticus