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Literature summary extracted from

  • Smith, K.A.; Salyers, A.A.
    Cell-associated pullulanase from Bacteroides thetaiotaomicron: cloning, characterization, and insertional mutagenesis to determine role in pullulan utilization (1989), J. Bacteriol., 171, 2116-2123.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli Bacteroides thetaiotaomicron

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 cytoplasm 80% soluble Bacteroides thetaiotaomicron 5737
-
3.2.1.41 membrane 18% membrane associated Bacteroides thetaiotaomicron 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 77000
-
 gel filtration Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Bacteroides thetaiotaomicron branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Bacteroides thetaiotaomicron
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Bacteroides thetaiotaomicron
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 of the recombinant protein Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Bacteroides thetaiotaomicron maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Bacteroides thetaiotaomicron maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Bacteroides thetaiotaomicron maltotriose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 monomer 1 * 77000, gel electrophoresis Bacteroides thetaiotaomicron

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Bacteroides thetaiotaomicron

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6.5
-
-
 Bacteroides thetaiotaomicron