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Literature summary extracted from

  • Zverlov, V.V.; Hertel, C.; Bronnenmeier, K.; Hroch, A.; Kellermann, J.; Schwarz, W.H.
    The thermostable alpha-L-rhamnosidase RamA of Clostridium stercorarium: biochemical characterization and primary structure of a bacterial alpha-L-rhamnoside hydrolase, a new type of inverting glycoside hydrolase (2000), Mol. Microbiol., 35, 173-179.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expression in Escherichia coli, gene has no homology to other glycoside hydrolases Thermoclostridium stercorarium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 alpha-L-rhamnose
-
 Thermoclostridium stercorarium
3.2.1.40 Cu2+
-
 Thermoclostridium stercorarium
3.2.1.40 Hg2+
-
 Thermoclostridium stercorarium
3.2.1.40 p-hydroxymercuribenzoate
-
 Thermoclostridium stercorarium
3.2.1.40 SDS
-
 Thermoclostridium stercorarium
3.2.1.40 Zn2+
-
 Thermoclostridium stercorarium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 95000
-
 SDS-PAGE, amino acid analysis Thermoclostridium stercorarium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.40 alpha-L-rhamnoside + H2O Thermoclostridium stercorarium alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Thermoclostridium stercorarium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40 of the recombinant protein Thermoclostridium stercorarium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 0.46
-
 hesperidin Thermoclostridium stercorarium
3.2.1.40 1.5
-
 naringin Thermoclostridium stercorarium
3.2.1.40 82
-
 p-nitrophenyl-alpha-L-rhamnopyranoside Thermoclostridium stercorarium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
-
 Thermoclostridium stercorarium 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Thermoclostridium stercorarium alpha-L-rhamnose + ?
-
 ?
3.2.1.40 hesperidin + H2O
-
 Thermoclostridium stercorarium hesperetin 7-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Thermoclostridium stercorarium beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 p-nitrophenyl alpha-L-rhamnoside + H2O
-
 Thermoclostridium stercorarium p-nitrophenol + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 dimer 2 * 95000, SDS-PAGE, amino acid analysis Thermoclostridium stercorarium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 alpha-L-rhamnosidase Ram A
-
 Thermoclostridium stercorarium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 60
-
-
 Thermoclostridium stercorarium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 42 68
-
 Thermoclostridium stercorarium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 7.5
-
-
 Thermoclostridium stercorarium