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Literature summary extracted from

  • Hashimoto, W.; Nankai, H.; Sato, N.; Kawai, S.; Murata, K.
    Characterization of alpha-L-rhamnosidase of Bacillus sp. GL1 responsible for the complete depolymerization of gellan (1999), Arch. Biochem. Biophys., 368, 56-60.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.40 pepstatin stabilizes Bacillus sp. (in: Bacteria)
3.2.1.40 phenylmethanesulfonyl fluorid stabilizes Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.40 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 100000
-
 native gradient PAGE, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.40 alpha-L-rhamnoside + H2O Bacillus sp. (in: Bacteria) alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
 ?
3.2.1.40 alpha-L-rhamnoside + H2O Bacillus sp. (in: Bacteria) GL1 alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Bacillus sp. (in: Bacteria)
-
 GL1
-
3.2.1.40 Bacillus sp. (in: Bacteria) GL1
-
 GL1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.40 culture filtrate
-
 Bacillus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Bacillus sp. (in: Bacteria) alpha-L-rhamnose + ?
-
 ?
3.2.1.40 alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Bacillus sp. (in: Bacteria) GL1 alpha-L-rhamnose + ?
-
 ?
3.2.1.40 p-nitrophenyl alpha-L-rhamnoside + H2O
-
 Bacillus sp. (in: Bacteria) p-nitrophenol + alpha-L-rhamnose
-
 ?
3.2.1.40 p-nitrophenyl alpha-L-rhamnoside + H2O
-
 Bacillus sp. (in: Bacteria) GL1 p-nitrophenol + alpha-L-rhamnose
-
 ?
3.2.1.40 rhamnosyl-glucose + H2O gellan as carbon-source Bacillus sp. (in: Bacteria) glucose + alpha-L-rhamnose
-
 ?
3.2.1.40 rhamnosyl-glucose + H2O gellan as carbon-source Bacillus sp. (in: Bacteria) GL1 glucose + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 monomer 1 * 100000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 55
-
 80% loss of activity after 10 min, pH 7 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 7
-
-
 Bacillus sp. (in: Bacteria)