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Literature summary extracted from

  • Manzanares, P.; de Graaff, L.H.; Visser, J.
    Purification and characterization of an alpha-L-rhamnosidase from Aspergillus niger (1997), FEMS Microbiol. Lett., 157, 279-283.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 alpha-L-rhamnose competitive Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 2.9
-
 p-nitrophenyl-alpha-L-rhamnopyranoside
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 85000
-
 gel filtration, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.40 alpha-L-rhamnoside + H2O Aspergillus niger alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.40 glycoprotein 12% N-glycosylated Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.40 commercial preparation
-
 Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 2-phenylethyl beta-D-rutinoside + H2O
-
 Aspergillus niger 2-phenylethyl beta-D-glucopyranoside + alpha-L-rhamnose
-
 ?
3.2.1.40 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
-
 Aspergillus niger 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Aspergillus niger alpha-L-rhamnose + ?
-
 ?
3.2.1.40 geranyl-beta-D-rutinoside + H2O
-
 Aspergillus niger geranyl-beta-D-glycoside + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O
-
 Aspergillus niger hesperetin 7-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Aspergillus niger beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 p-nitrophenyl alpha-L-rhamnoside + H2O
-
 Aspergillus niger p-nitrophenol + alpha-L-rhamnose
-
 ?
3.2.1.40 p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Aspergillus niger p-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 rutin + H2O
-
 Aspergillus niger 3-glycosylquercetin + alpha-L-rhamnose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 65
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 40
-
 no loss of activity after 4 h Aspergillus niger
3.2.1.40 50
-
 15% loss of activity after 4 h Aspergillus niger
3.2.1.40 60
-
 40% loss of activity after 1 h Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 4.5 5
-
 Aspergillus niger