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Literature summary extracted from

  • Mutter, M.; Beldman, G.; Schols, H.A.; Voragen, A.G.
    Rhamnogalacturonan alpha-L-rhamnopyranohydrolase. A novel enzyme specific for the terminal nonreducing rhamnosyl unit in rhamnogalacturonan regions of pectin (1994), Plant Physiol., 106, 241-250.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 Sodium acetate above 0.3 M , at pH 5 Aspergillus aculeatus
3.2.1.174 Sodium acetate 0.1 M slowly inactivates the enzyme: at 0.5 M 33% residual activity, at 1.1 M 10% residual activity Aspergillus aculeatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 87000
-
 gel filtration, SDS-PAGE Aspergillus aculeatus
3.2.1.174 84000
-
 x * 84000, SDS-PAGE Aspergillus aculeatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.40 alpha-L-rhamnoside + H2O Aspergillus aculeatus alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Aspergillus aculeatus
-
-
-
3.2.1.174 Aspergillus aculeatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Aspergillus aculeatus
3.2.1.174
-
 Aspergillus aculeatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.40 commercial preparation
-
 Aspergillus aculeatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
-
 Aspergillus aculeatus 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Aspergillus aculeatus alpha-L-rhamnose + ?
-
 ?
3.2.1.40 hesperidin + H2O
-
 Aspergillus aculeatus hesperetin 7-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 high-methoxyl pectin + H2O
-
 Aspergillus aculeatus pyranoside + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Aspergillus aculeatus beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 p-nitrophenyl alpha-L-rhamnoside + H2O
-
 Aspergillus aculeatus p-nitrophenol + alpha-L-rhamnose
-
 ?
3.2.1.40 polygalacturonic acid + H2O
-
 Aspergillus aculeatus D-galacturonic acid
-
 ?
3.2.1.174 rhamnogalacturonan I oligosaccharides with D-galacturonic acid at the reducing end and L-rhamnopyranose at the nonreducing end + H2O alpha-L-Rha-(1->4)-[alpha-D-GalA-(1->2)-alpha-L-Rha]n-(1->4)-alpha-D-GalA. The enzyme is hindered when the terminal Rha residue is substituted at the 4-position by a beta-D-galactose. No particular preference of the enzyme for low or high molecular mass rhamnogalacturonan fragments Aspergillus aculeatus beta-L-rhamnopyranose + shortened rhamnogalacturonan I oligosaccharides with beta-D-galacturonic acid at the reducing end and at the nonreducing end
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.174 ? x * 84000, SDS-PAGE Aspergillus aculeatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 More similar enzyme: RG-rhamnohydrolase Aspergillus aculeatus
3.2.1.40 pnp-rhamnohydrolase
-
 Aspergillus aculeatus
3.2.1.174 RG alpha-L-rhamnopyranohydrolase
-
 Aspergillus aculeatus
3.2.1.174 RG-rhamnohydrolase
-
 Aspergillus aculeatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 60
-
-
 Aspergillus aculeatus
3.2.1.174 60
-
 sodium acetate buffer Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 40
-
 in McIlvaine buffer, pH 5, after 30 min, 50% loss of activity Aspergillus aculeatus
3.2.1.40 50
-
 in sodium acetate buffer, pH 5, after 1 h no loss of activity at Aspergillus aculeatus
3.2.1.174 60
-
 stable for 1 h up to temperatures of 60°C in 50 mM sodium acetate buffer (pH 5) Aspergillus aculeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 5.5 6
-
 Aspergillus aculeatus
3.2.1.174 4
-
 McIlvaine buffer Aspergillus aculeatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 3 8
-
 Aspergillus aculeatus
3.2.1.174 3 8 stable Aspergillus aculeatus