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Literature summary extracted from

  • Manners, D.J.; Wilson, G.
    Studies on beta-glucanases. Some properties of a bacterial endo-beta-(1-3)-glucanase system (1973), Biochem. J., 135, 11-18.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.2.1.6 about 66% of activity lost after dialysis against distilled water at 2°C for 18 h Flavobacterium sp.
3.2.1.6 stabilization of activity at 37°C for 3 h by bovine serum albumin, no effect for longer periods Flavobacterium sp.
3.2.1.39 very unstable during dilution and dialysis, addition of Ca2+ and bovine serum albumin partly restores activity Flavobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.39 extracellular
-
 Flavobacterium sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.6 Ca2+ 32% increase of activity by 10 mM Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 16600
-
 gel filtration Flavobacterium sp.
3.2.1.39 16000
-
 gel filtration Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Flavobacterium sp.
-
 Cytophaga
-
3.2.1.39 Flavobacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, continuous-curtain electrophoresis Flavobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 culture filtrate
-
 Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 0.33
-
-
 Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 beta-glucan + H2O
-
 Flavobacterium sp. laminaridextrins D-glucose + cellobiose ?
3.2.1.6 carboxymethylpachyman + H2O
-
 Flavobacterium sp. D-glucose + carboxymethyl-(1-3,1-4)-beta-D-glucan
-
 ?
3.2.1.6 cellobiose + H2O
-
 Flavobacterium sp. 2 D-glucose
-
 ?
3.2.1.6 cellodextrin + H2O
-
 Flavobacterium sp. cellobiose + cellotriose + higher oligosaccharides
-
 ?
3.2.1.6 laminaribiose + H2O
-
 Flavobacterium sp. D-glucose
-
 ?
3.2.1.6 laminarin + H2O
-
 Flavobacterium sp. D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
 ?
3.2.1.6 lichenin + H2O
-
 Flavobacterium sp. beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
 ?
3.2.1.6 pachyman + H2O
-
 Flavobacterium sp. laminarisaccharides
-
 ?
3.2.1.39 (1-3:1-4)-beta-D-glucan + H2O lichenan Flavobacterium sp. ?
-
 ?
3.2.1.39 (1-3:1-4)-beta-D-glucan + H2O release of cellobiose as main product Flavobacterium sp. ?
-
 ?
3.2.1.39 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O low activity Flavobacterium sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.39 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Flavobacterium sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.39 beta-glucan + H2O from barley Flavobacterium sp. ?
-
 ?
3.2.1.39 beta-glucan + H2O beta-1,3- and beta-1,4-linkages Flavobacterium sp. ?
-
 ?
3.2.1.39 beta-glucan + H2O major product cellobiose Flavobacterium sp. ?
-
 ?
3.2.1.39 laminarin + H2O release of laminarisaccharides with polymerization degree from 1-7 Flavobacterium sp. D-glucose + ?
-
 ?
3.2.1.39 O-carboxymethyl-pachyman + H2O release of laminarisaccharides ranging in polymerization degree from 1-7 Flavobacterium sp. ?
-
 ?
3.2.1.39 pachyman + H2O release of laminarisaccharides in polymerization degree form 1-7 Flavobacterium sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 endo-beta-1,3-glucanase
-
 Flavobacterium sp.
3.2.1.39 endo-beta-1,3-glucanase
-
 Flavobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 37
-
 with barley beta-glucan as substrate, 26% of activity after 30 min, no activity after 180 min Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5
-
-
 Flavobacterium sp.
3.2.1.39 5
-
-
 Flavobacterium sp.