Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nagasaki, S.; Nishioka, Y.; Mori, H.; Yamamato, S.
    Purification and properties of lytic beta-1,3-glucanase from Flavobacterium dormitator var. glucanolyticae (1976), Agric. Biol. Chem., 40, 1059-1067.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.39 Sodium diethyldithiocarbamate 10% activation at 1 mM Flavobacterium dormitator

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 4-chloromercuribenzoate 45% inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 Cu2+ strong inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 EDTA nearly complete inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 Hg2+ 36% inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 Iodine 30% inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 K3Fe(CN)6 slight inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 KMnO4 complete inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 NaAsO2 slight inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 NaN3 slight inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 Ni2+ slight inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 Pb2+ 59% inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 Semicarbazide slight inhibition at 1 mM Flavobacterium dormitator
3.2.1.39 Zn2+ 25% inhibition at 1 mM Flavobacterium dormitator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 Laminarin value in mg/ml Flavobacterium dormitator
3.2.1.39 additional information
-
 Glucan value in mg/ml: 1.8 Flavobacterium dormitator
3.2.1.39 additional information
-
 Laminarihexaose value in mg/ml: 1.18 Flavobacterium dormitator
3.2.1.39 additional information
-
 laminaritetraose value in mg/ml: 2.0 Flavobacterium dormitator
3.2.1.39 additional information
-
 Laminarin 0.26 Flavobacterium dormitator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.39 extracellular
-
 Flavobacterium dormitator
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.39 Mg2+ 15% activation at 1 mM Flavobacterium dormitator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 17000 20000 SDS-PAGE, gel filtration Flavobacterium dormitator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 cell wall glucan + H2O Flavobacterium dormitator living yeast cells reducing sugar + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Flavobacterium dormitator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 partial Flavobacterium dormitator

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 culture filtrate
-
 Flavobacterium dormitator
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 10.1
-
-
 Flavobacterium dormitator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 (1-3:1-4)-beta-D-glucan + H2O lichenan Flavobacterium dormitator ?
-
 ?
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc + H2O releases laminaribiose and glucose Flavobacterium dormitator ?
-
 ?
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc + H2O laminaritriose Flavobacterium dormitator ?
-
 ?
3.2.1.39 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Flavobacterium dormitator beta-D-glucose + D-glucose
-
 ?
3.2.1.39 beta-D-glucan + H2O
-
 Flavobacterium dormitator ?
-
 ?
3.2.1.39 beta-glucan + H2O from yeast Flavobacterium dormitator ?
-
 ?
3.2.1.39 cell wall glucan + H2O living yeast cells Flavobacterium dormitator reducing sugar + ?
-
 ?
3.2.1.39 laminarihexaose + H2O
-
 Flavobacterium dormitator ?
-
 ?
3.2.1.39 laminarin + H2O major products are glucose, laminaribiose, laminaritriose, laminaritetraose and higher liminarioligosaccharides Flavobacterium dormitator D-glucose + ?
-
 ?
3.2.1.39 pachyman + H2O
-
 Flavobacterium dormitator ?
-
 ?
3.2.1.39 periodate-oxidized laminarin + H2O
-
 Flavobacterium dormitator ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 50
-
-
 Flavobacterium dormitator

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 60
-
 complete loss of activity above Flavobacterium dormitator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 6
-
-
 Flavobacterium dormitator

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 6 9 stable within Flavobacterium dormitator