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Literature summary extracted from

  • Reichelt, B.Y.; Fleet, G.H.
    Isolation, properties, function, and regulation of endo-(1-3)-beta-glucanases in Schizosaccharomyces pombe (1981), J. Bacteriol., 147, 1085-1094.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.39 Trypsin stimulation of release from cell walls Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 Laminarin slight inhibition above 1 mg/ml for glucanase I, above 0.5 mg/ml inhibitory for glucanase II Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 Laminarin value in mg/ml Schizosaccharomyces pombe
3.2.1.39 additional information
-
 Laminarin 0.15 , glucanase I Schizosaccharomyces pombe
3.2.1.39 additional information
-
 Laminarin 0.69 glucanase II Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.39 cell wall two different forms, only present in conjugating and sporulating cells, not present in vegetative cells Schizosaccharomyces pombe 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 75000
-
 SDS-PAGE, gel filtration, glucanase II Schizosaccharomyces pombe
3.2.1.39 78500
-
 1 * 78500, 1 * 82000, glucanase II, SDS-PAGE Schizosaccharomyces pombe
3.2.1.39 82000
-
 1 * 78500, 1 * 82000, glucanase II, SDS-PAGE Schizosaccharomyces pombe
3.2.1.39 160500
-
 SDS-PAGE, gel filtration, glucanase I Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 cell wall glucan + H2O Schizosaccharomyces pombe involved in modification and limited degradation of the cell wall during cell life cycle reducing sugar + ?
-
 ?
3.2.1.39 cell wall glucan + H2O Schizosaccharomyces pombe cell wall autolysis reducing sugar + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 3step chromatography for glucanase I, 4step chromatography for glucanase II Schizosaccharomyces pombe

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 cell culture enzyme activation due to factors produced during conjugation and sporulation Schizosaccharomyces pombe
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 1.89
-
 for glucanase I Schizosaccharomyces pombe
3.2.1.39 5.89
-
 for glucanase II Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 cell wall glucan + H2O involved in modification and limited degradation of the cell wall during cell life cycle Schizosaccharomyces pombe reducing sugar + ?
-
 ?
3.2.1.39 cell wall glucan + H2O cell wall autolysis Schizosaccharomyces pombe reducing sugar + ?
-
 ?
3.2.1.39 laminarin + H2O specifically hydrolyzes (1-3)-beta-glycosidic linkages, predominant products are laminaribiose and laminaritriose, minor amounts of glucose and higher oligosaccharides Schizosaccharomyces pombe D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 dimer 1 * 78500, 1 * 82000, glucanase II, SDS-PAGE Schizosaccharomyces pombe
3.2.1.39 monomer 1 * 75000, glucanase I, SDS-PAGE, gel filtration Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-(1-3)-beta-glucanase two different forms, glucanase I and glucanase II Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 30
-
 both glucanases I and II Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5
-
 both forms, glucanase I and glucanase II Schizosaccharomyces pombe