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Literature summary extracted from

  • Schwarz, W.; Bronnenmeier, K.; Staudenbauer, W.L.
    Molecular cloning of Clostridium thermocellum genes involved in beta-glucan degradation in bacteriophage lambda (1985), Biotechnol. Lett., 7, 859-864.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression in Escherichia coli Acetivibrio thermocellus
3.2.1.39 expression in Escherichia coli Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Acetivibrio thermocellus
-
-
-
3.2.1.39 Acetivibrio thermocellus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 0.23
-
 carboxymethylcellulose as substrate Acetivibrio thermocellus
3.2.1.6 0.4
-
 lichenin as substrate Acetivibrio thermocellus
3.2.1.6 2.83
-
 laminarin as substrate Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 carboxymethylcellulose + H2O
-
 Acetivibrio thermocellus D-glucose + ?
-
 ?
3.2.1.6 laminarin + H2O
-
 Acetivibrio thermocellus D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose reducing sugars ?
3.2.1.6 lichenin + H2O
-
 Acetivibrio thermocellus beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers laminaripentaose + D-glucose ?
3.2.1.39 laminarin + H2O
-
 Acetivibrio thermocellus D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 laminarinase
-
 Acetivibrio thermocellus
3.2.1.39 beta-1,3-endoglucanase
-
 Acetivibrio thermocellus