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Literature summary extracted from

  • Mauch, F.; Hadwiger, L.A.; Boller, T.
    Antifungal hydrolases in pea tissue. I. Purification and characterization of two chitinases and two beta-1,3-glucanases differentially regulated during development and in response to fungal infection (1988), Plant Physiol., 87, 325-333.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 33100
-
 x * 33100, isoenzyme I, SDS-PAGE, x * 36200, isoenzyme II, SDS-PAGE, x * 39000, isoenzyme III, SDS-PAGE Pisum sativum
3.2.1.14 36200
-
 x * 33100, isoenzyme I, SDS-PAGE, x * 36200, isoenzyme II, SDS-PAGE, x * 39000, isoenzyme III, SDS-PAGE Pisum sativum
3.2.1.14 39000
-
 x * 33100, isoenzyme I, SDS-PAGE, x * 36200, isoenzyme II, SDS-PAGE, x * 39000, isoenzyme III, SDS-PAGE Pisum sativum
3.2.1.39 33500
-
 isoform G1, SDS-PAGE Pisum sativum
3.2.1.39 34300
-
 isoform G2, SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Pisum sativum
-
-
-
3.2.1.39 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 2step chromatography Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 pod increase of two isoenzymes, G1 and G2, after inoculation by Fusarium solani Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 12.6
-
 isoenzyme II Pisum sativum
3.2.1.14 18.6
-
 isoenzyme I Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O [3H]chitin, isoenzymes I and II show lysozyme activity Pisum sativum N,N-diacetylchitobiose + N-acetylglucosamine
-
 ?
3.2.1.39 laminarin + H2O accumulation of the enzyme after inoculation with fungi, involvement in pathogenesis in relation to active plant defense Pisum sativum D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 33100, isoenzyme I, SDS-PAGE, x * 36200, isoenzyme II, SDS-PAGE, x * 39000, isoenzyme III, SDS-PAGE Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4.2
-
 isoenzyme I Pisum sativum
3.2.1.14 4.7
-
 isoenzymes II and III Pisum sativum
3.2.1.39 5.1
-
 isoform G1 Pisum sativum
3.2.1.39 5.4
-
 isoform G2 Pisum sativum