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Literature summary extracted from

  • Hrmova, M.; Fincher, G.B.
    Purification and properties of three (1-3)-beta-D-glucanase isoenzymes from young leaves of barley (Hordeum vulgare) (1993), Biochem. J., 289, 453-461.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 0.17
-
Laminarin isoform GI Hordeum vulgare
3.2.1.39 0.18
-
Laminarin isoform GIII Hordeum vulgare
3.2.1.39 0.21
-
Laminarin isoform GII Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 32000
-
isoforms GI, GII and GIII, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Hordeum vulgare
-
barley
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.39 glycoprotein isoform GIII, 12-17% carbohydrate content, w/w Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 4-5step chromatography Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 leaf
-
Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 40.5
-
isoform GIII Hordeum vulgare
3.2.1.39 240.5
-
isoform GII Hordeum vulgare
3.2.1.39 281.5
-
isoform GI Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 beta-glucan + H2O low activity Hordeum vulgare ?
-
?
3.2.1.39 beta-glucan + H2O from yeast Hordeum vulgare ?
-
?
3.2.1.39 laminarin + H2O highest rates from Laminaria digitata, major products are laminaribiose and laminaritriose for isoforms GII and GIII, GI yields predominantly (1-3)-beta-D-oligoglucosides greater than 4 units Hordeum vulgare D-glucose + ?
-
?
3.2.1.39 O-carboxymethyl-(1,3:1,6)-beta-D-glucan + H2O from yeast, low activity Hordeum vulgare ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 (1-3)-beta-D-glucanase three isoforms, GI, GII and GIII Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 4.8
-
isoforms GI, GII and GIII Hordeum vulgare

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 5
-
below less activity for isoform GI than for GII or GIII Hordeum vulgare
3.2.1.39 6
-
isoforms GI and GII retain 17-27% activity, GIII retaines 66% activity Hordeum vulgare