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Literature summary extracted from

  • Churngchow, N.; Suntaro, A.; Wititsuwannnakul, R.
    beta-1,3-Glucanase isoenzymes from the latex of Hevea brasiliensis (1995), Phytochemistry, 39, 505-509.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 Laminarin value in mg/ml Hevea brasiliensis
3.2.1.39 additional information
-
 Laminarin 1.24, isoform GI Hevea brasiliensis
3.2.1.39 additional information
-
 Laminarin 1.33 isoform GII Hevea brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 32000
-
 isoform GI, SDS-PAGE, gel filtration Hevea brasiliensis
3.2.1.39 35000
-
 isoform GII, SDS-PAGE, gel filtration Hevea brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Hevea brasiliensis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.39 glycoprotein isoform GII, glycosylated with glucose and/or mannose residues Hevea brasiliensis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 2step chromatography Hevea brasiliensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 latex two isoforms, GI and GII Hevea brasiliensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 0.01
-
 isoform GII Hevea brasiliensis
3.2.1.39 0.013
-
 isoform GI Hevea brasiliensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O hydrolysis at highest rates by isoform GI, lower hydrolysis by isoform GII, enzyme requires a long run of contiguous beta-1,3-D-glucosidic linkages, where glucosyl units neither substituted nor branched Hevea brasiliensis D-glucose + ?
-
 ?
3.2.1.39 O-carboxymethyl-pachyman + H2O faster hydrolysis by isoform GII than by isoform GI, isoform GII may play a role in response to fungal infection Hevea brasiliensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 monomer 1 * 32000, isoform GI, SDS-PAGE, gel filtration Hevea brasiliensis
3.2.1.39 monomer 1 * 35000, isoform GII, SDS-PAGE, gel filtration Hevea brasiliensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 60
-
 stable up to, rapid inactivation above Hevea brasiliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 4.5
-
 isoform GI Hevea brasiliensis
3.2.1.39 5
-
 isoform GII Hevea brasiliensis