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Literature summary extracted from

  • Akiyama, T.; Shibuya, N.; Hrmova, M.; Fincher, G.B.
    Purification and characterization of a (1-3)-beta-D-glucan endohydrolase from rice (Oryza sativa) bran (1997), Carbohydr. Res., 297, 365-374.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 0.04
-
 Laminarin
-
 Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 29000
-
 SDS-PAGE, gel filtration Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 4step chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 seed coat
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 500
-
-
 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 (1-3:1-4)-beta-D-glucan + H2O very low activity Oryza sativa ?
-
 ?
3.2.1.39 (1-3:1-4)-beta-D-glucan + H2O lichenan Oryza sativa ?
-
 ?
3.2.1.39 curdlan + H2O from Alcaligenes faecalis Oryza sativa ?
-
 ?
3.2.1.39 curdlan + H2O insoluble reduced 1,3-beta-glucan Oryza sativa ?
-
 ?
3.2.1.39 laminarin + H2O from Laminaria digitata, lower rates are from Laminaria hyperborea and Eisenia bicyclis, best substrates are unbranched (1-3)-beta-D-glucans Oryza sativa D-glucose + ?
-
 ?
3.2.1.39 O-carboxymethyl-(1,3:1,6)-beta-D-glucan + H2O from yeast, low activity Oryza sativa ?
-
 ?
3.2.1.39 O-carboxymethyl-pachyman + H2O unbranched (1-3)-beta-glucan from Poria cocos Oryza sativa ?
-
 ?
3.2.1.39 schizophyllan + H2O from Schizophyllum commune, low activity Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 monomer 1 * 29000, SDS-PAGE, gel filtration Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 (1-3)-beta-D-glucan endohydrolase
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 60
-
-
 Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 60 70 activity rapidly lost Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5
-
-
 Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 3.5 6 rapid inactivation below and above Oryza sativa