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Literature summary extracted from

  • Colas, B.
    Some physicochemical and structural properties of two beta-fucosidases from Achatina balteata (1978), Biochim. Biophys. Acta, 527, 150-158.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.38 repeated freezing and thawing cycles don't affect fucosidase II, 30% loss of activity for beta-fucosidase I after 3 cycles Achatina balteata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.38 Urea activity of fucosidase I more rapidly lost than that of fucosidase II in 6 M urea Achatina balteata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.38 extracellular
-
 Achatina balteata
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.38 105000 120000 beta-fucosidase II, gel filtration, ultracentrifugation Achatina balteata
3.2.1.38 110000
-
 1 * 110000, beta-fucosidase II, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata
3.2.1.38 170000
-
 1 * 170000 + 1 * 190000, beta-fucosidase I, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata
3.2.1.38 190000
-
 1 * 170000 + 1 * 190000, beta-fucosidase I, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata
3.2.1.38 300000 360000 beta-fucosidase I, gel filtration, ultracentrifugation Achatina balteata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.38 Achatina balteata
-
 african snail
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.38 glycoprotein contains galactose, glucose, mannose, fucose, 15.5%, w/w, for beta-fucosidase I, 7.5%, w/w, for beta-fucosidase II, contains hexosamines, 6.1%, w/w, for beta-fucosidase I, 5.2%, w/w, for beta-fucosidase II, no sialic acid found Achatina balteata

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.38 to homogeneity Achatina balteata

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.38 50% recovery for beta-fucosidase II, 25% recovery for beta-fucosidase I after treatment with 6 M urea Achatina balteata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.38 digestive juice two forms, beta-fucosidase I and beta-fucosidase II Achatina balteata
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.38 4°C, 50 mM sodium acetate, pH 5.4, several months Achatina balteata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.38 p-nitrophenyl-beta-D-fucoside + H2O
-
 Achatina balteata p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-galactoside + H2O
-
 Achatina balteata p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-glucoside + H2O
-
 Achatina balteata p-nitrophenol + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.38 dimer 1 * 170000 + 1 * 190000, beta-fucosidase I, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata
3.2.1.38 monomer 1 * 110000, beta-fucosidase II, SDS-PAGE, gel filtration, ultracentrifugation Achatina balteata

Synonyms

EC Number Synonyms Comment Organism
3.2.1.38 beta-fucosidase I
-
 Achatina balteata
3.2.1.38 beta-fucosidase II
-
 Achatina balteata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.38 53
-
 beta-fucosidase I, 60% loss of activity Achatina balteata
3.2.1.38 55
-
 beta-fucosidase II, 50% loss of activity Achatina balteata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.38 5 7.5 beta-fucosidase II Achatina balteata
3.2.1.38 5.5 7 beta-fucosidase I Achatina balteata