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Literature summary extracted from
- Purification and properties of thermostable beta-xylosidase from immature stalks of Saccharum officinarum L. (sugar cane) (1982), J. Biochem., 92, 1873-1881.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.37 | xylose protects against thermal inactivation | Saccharum officinarum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.37 | Ag+ | non-competitive inhibition | Saccharum officinarum |  |
| 3.2.1.37 | beta-D-xylose | competitive inhibition | Saccharum officinarum | |
| 3.2.1.37 | Hg2+ | competitive inhibition | Saccharum officinarum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.37 | 2.05 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Saccharum officinarum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 62000 | - |
SDS-PAGE, gel filtration | Saccharum officinarum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.37 | Saccharum officinarum | - |
sugar cane | - |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 3.2.1.37 | sensitive to photooxidation | Saccharum officinarum |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | glycoprotein | 13.5% carbohydrate moiety, arabinose, galactose residues | Saccharum officinarum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.37 | to homogeneity by heat treatment, chromatography steps | Saccharum officinarum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.37 | stem | - |
Saccharum officinarum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 3.8 | - |
- |
Saccharum officinarum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.37 | o-nitrophenyl beta-D-xylopyranoside + H2O | - |
Saccharum officinarum | o-nitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.37 | p-nitrophenyl beta-D-xylopyranoside + H2O | more rapidly hydrolyzed than o-nitrophenyl beta-D-xylopyranoside | Saccharum officinarum | p-nitrophenol + beta-D-xylopyranose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | monomer | 1 * 62000, SDS-PAGE, gel filtration | Saccharum officinarum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 75 | - |
- |
Saccharum officinarum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 70 | - |
fully active within 1 h in the presence of 0.1% D-xylose | Saccharum officinarum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 4.8 | - |
- |
Saccharum officinarum |
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Release 2023.1 (January 2023)
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