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Literature summary extracted from

  • Rodionova, N.A.; Tavobilov, I.M.; Bezborodov, A.M.
    beta-xylosidase from Aspergillus niger 15: Purification and properties (1983), J. Appl. Biochem., 5, 300-312.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 beta-D-xylose competitive inhibition Aspergillus niger
3.2.1.37 p-chloromercuribenzoate
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.23
-
 p-nitrophenyl beta-D-xylopyranoside
-
 Aspergillus niger
3.2.1.37 0.67
-
 xylobiose
-
 Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 122000
-
 2 * 122000, SDS-PAGE Aspergillus niger
3.2.1.37 253000
-
 gel filtration, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 xylobiose + H2O Aspergillus niger transxylosylation activity beta-D-xylose + D-xylose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 to homogeneity by 4step chromatography Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 cell culture
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 35.22
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Aspergillus niger 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.37 2-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O sophorose, low activity Aspergillus niger beta-D-glucose + D-glucose
-
 ?
3.2.1.37 3-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O laminaribiose, low activity Aspergillus niger beta-D-glucose + D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucopyranoside + H2O low activity Aspergillus niger 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.37 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose, 30% activity Aspergillus niger beta-D-glucose + D-glucose
-
 ?
3.2.1.37 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose, low activity Aspergillus niger beta-D-glucose + D-glucose
-
 ?
3.2.1.37 methyl-beta-D-xyloside + H2O very low activity Aspergillus niger beta-D-xylose + methanol
-
 ?
3.2.1.37 p-nitrophenyl alpha-L-arabinoside + H2O low activity Aspergillus niger p-nitrophenol + alpha-L-arabinose
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 phenyl 4-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O 25% activity Aspergillus niger phenyl beta-D-glucopyranoside + D-glucose
-
 ?
3.2.1.37 phenyl beta-D-glucopyranoside + H2O low activity Aspergillus niger phenol + D-glucopyranose
-
 ?
3.2.1.37 xylobiose + H2O transxylosylation activity Aspergillus niger beta-D-xylose + D-xylose
-
 ?
3.2.1.37 xylopentaose + H2O
-
 Aspergillus niger beta-D-xylose + ?
-
 ?
3.2.1.37 xylotriose + H2O
-
 Aspergillus niger beta-D-xylose + xylobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 dimer 2 * 122000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 70
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 50
-
 stable up to 1 h Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 3.8 4
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 3 8 stable within Aspergillus niger