BRENDA - Enzyme Database

Purification and some properties of beta-xylosidase from Trichoderma viride

Matsuo, M.; Yasui, T.; Agric. Biol. Chem. 48, 1845-1852 (1984)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
Hg2+
strong inhibition at 1 mM
Trichoderma viride
3.2.1.37
N-bromosuccinimide
strong inhibition at 1 mM
Trichoderma viride
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.37
extracellular
-
Trichoderma viride
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.37
101000
102000
SDS-PAGE, gel filtration
Trichoderma viride
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.37
xylobiose + H2O
Trichoderma viride
-
beta-D-xylose + D-xylose
-
Trichoderma viride
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.37
Trichoderma viride
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.37
glycoprotein
4.5% carbohydrate moiety
Trichoderma viride
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.37
to homogeneity by chromatography and isoelectric focusing
Trichoderma viride
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.37
cell culture
-
Trichoderma viride
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.37
10.8
-
-
Trichoderma viride
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.37
o-chlorophenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
o-chlorophenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
o-methoxyphenyl beta-D-xyloside
-
136304
Trichoderma viride
o-methoxyphenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
o-methylphenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
o-methylphenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
o-nitrophenyl-beta-D-xyloside + H2O
-
136304
Trichoderma viride
o-nitrophenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
p-methoxyphenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
p-methoxyphenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
p-methylphenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
p-methylphenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
p-nitrophenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
p-nitrophenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
phenyl beta-D-xyloside + H2O
with retention of configuration
136304
Trichoderma viride
phenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
xylobiose + H2O
-
136304
Trichoderma viride
beta-D-xylose + D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
xylopentaose + H2O
-
136304
Trichoderma viride
beta-D-xylose + ?
-
136304
Trichoderma viride
?
3.2.1.37
xylotetraose + H2O
-
136304
Trichoderma viride
beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
xylotriose + H2O
-
136304
Trichoderma viride
beta-D-xylose + xylobiose
-
136304
Trichoderma viride
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.37
monomer
1 * 101000-102000, SDS-PAGE, gel filtration
Trichoderma viride
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
55
-
-
Trichoderma viride
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
55
-
stable up to
Trichoderma viride
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
4.5
-
-
Trichoderma viride
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.37
3
4
stable for 30 min at 50°C
Trichoderma viride
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
Hg2+
strong inhibition at 1 mM
Trichoderma viride
3.2.1.37
N-bromosuccinimide
strong inhibition at 1 mM
Trichoderma viride
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.37
extracellular
-
Trichoderma viride
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.37
101000
102000
SDS-PAGE, gel filtration
Trichoderma viride
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.37
xylobiose + H2O
Trichoderma viride
-
beta-D-xylose + D-xylose
-
Trichoderma viride
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.37
glycoprotein
4.5% carbohydrate moiety
Trichoderma viride
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.37
to homogeneity by chromatography and isoelectric focusing
Trichoderma viride
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.37
cell culture
-
Trichoderma viride
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.37
10.8
-
-
Trichoderma viride
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.37
o-chlorophenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
o-chlorophenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
o-methoxyphenyl beta-D-xyloside
-
136304
Trichoderma viride
o-methoxyphenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
o-methylphenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
o-methylphenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
o-nitrophenyl-beta-D-xyloside + H2O
-
136304
Trichoderma viride
o-nitrophenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
p-methoxyphenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
p-methoxyphenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
p-methylphenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
p-methylphenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
p-nitrophenyl beta-D-xyloside + H2O
-
136304
Trichoderma viride
p-nitrophenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
phenyl beta-D-xyloside + H2O
with retention of configuration
136304
Trichoderma viride
phenol + beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
xylobiose + H2O
-
136304
Trichoderma viride
beta-D-xylose + D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
xylopentaose + H2O
-
136304
Trichoderma viride
beta-D-xylose + ?
-
136304
Trichoderma viride
?
3.2.1.37
xylotetraose + H2O
-
136304
Trichoderma viride
beta-D-xylose
-
136304
Trichoderma viride
?
3.2.1.37
xylotriose + H2O
-
136304
Trichoderma viride
beta-D-xylose + xylobiose
-
136304
Trichoderma viride
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.37
monomer
1 * 101000-102000, SDS-PAGE, gel filtration
Trichoderma viride
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
55
-
-
Trichoderma viride
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
55
-
stable up to
Trichoderma viride
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
4.5
-
-
Trichoderma viride
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.37
3
4
stable for 30 min at 50°C
Trichoderma viride