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Literature summary extracted from

  • Lappalainen, A.
    Purification and characterization of xylanolytic enzymes from Trichoderma reesei (1986), Biotechnol. Appl. Biochem., 8, 437-448.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Trichoderma reesei
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 23000
-
 x * 23000, xylanase II, SDS-PAGE Trichoderma reesei
3.2.1.8 32000
-
 x * 32000, xylanase I, SDS-PAGE Trichoderma reesei
3.2.1.37 90000
-
 SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 xylan + H2O Trichoderma reesei low activity beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O Trichoderma reesei
-
 beta-D-xylose + D-xylose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Trichoderma reesei
-
-
-
3.2.1.37 Trichoderma reesei
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein xylanase I contains 14% carbohydrate, xylanase II contains 8% carbohydrate Trichoderma reesei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 xylanase I and II Trichoderma reesei
3.2.1.37 partial Trichoderma reesei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 cell culture
-
 Trichoderma reesei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 1,4-beta-D-xylan + H2O insoluble birch xylan Trichoderma reesei additional information xylobiose and higher xylo-oligomers as main hydrolysis products ?
3.2.1.8 1,4-beta-D-xylan + H2O oat hulls xylan Trichoderma reesei additional information xylobiose and higher xylo-oligomers as main hydrolysis products ?
3.2.1.8 beta-glucan + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.8 carboxymethylcellulose + H2O slight activity with xylanase I, no activity with xylanase II Trichoderma reesei ?
-
 ?
3.2.1.8 glucomannan + H2O slight activity with xylanase I, no activity with xylanase II Trichoderma reesei ?
-
 ?
3.2.1.8 laminarin + H2O xylanase I Trichoderma reesei ?
-
 ?
3.2.1.8 O-acetyl-4-O-glucuronoxylan + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.8 pachyman + H2O slight activity with xylanase I, no activity with xylanase II Trichoderma reesei ?
-
 ?
3.2.1.8 pustulan + H2O slight activity with xylanase I, no activity with xylanase II Trichoderma reesei ?
-
 ?
3.2.1.8 starch + H2O soluble starch Trichoderma reesei ?
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xyloside + H2O
-
 Trichoderma reesei p-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 xylan + H2O low activity Trichoderma reesei beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Trichoderma reesei beta-D-xylose + D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 32000, xylanase I, SDS-PAGE Trichoderma reesei
3.2.1.8 ? x * 23000, xylanase II, SDS-PAGE Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 40
-
 20 h, 10% loss of activity of xylanase I, xylanase II is stable Trichoderma reesei
3.2.1.8 50
-
 20 h, 27% loss of activity of xylanase I and II Trichoderma reesei
3.2.1.8 60
-
 20 h, 84% loss of activity of xylanase I, 76% loss of activity of xylanase II Trichoderma reesei
3.2.1.8 70
-
 20 h, complete inactivation Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 4 5 xylanase I and II Trichoderma reesei