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Literature summary extracted from

  • Poutanen, K.; Puls, J.
    Characteristics of Trichoderma reesei beta-xylosidase and its use in the hydrolysis of solubilized xylans (1988), Appl. Microbiol. Biotechnol., 28, 425-432.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 beta-D-xylose 25% competitive inhibition at 50 mM Trichoderma reesei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Trichoderma reesei
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 100000
-
 SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 xylan + H2O Trichoderma reesei
-
 beta-D-xylose + ?
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O Trichoderma reesei
-
 beta-D-xylose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Trichoderma reesei
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein 7.5% carbohydrate moiety Trichoderma reesei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 to homogeneity by 2step chromatography Trichoderma reesei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 cell culture
-
 Trichoderma reesei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 p-nitrophenyl alpha-L-arabinofuranoside + H2O low affinity, 70% activity of that for p-nitrophenyl beta-D-xylopyranose Trichoderma reesei p-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Trichoderma reesei p-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 xylan + H2O
-
 Trichoderma reesei beta-D-xylose + ?
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O
-
 Trichoderma reesei beta-D-xylose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 monomer 1 * 100000, SDS-PAGE Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 60
-
-
 Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 55
-
 stable up to Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 4
-
-
 Trichoderma reesei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 3 6 stable for 24 h at 45°C Trichoderma reesei
3.2.1.37 6
-
 50% activity Trichoderma reesei