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Literature summary extracted from
- Xylanases and beta-xylosidase of Trichoderma lignorum (1988), Methods Enzymol., 160, 662-671.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.37 | beta-D-xylose | 60% inhibition at 10 mM | Trichoderma viride |  |
| 3.2.1.37 | glucose | 16% inhibition at 10 mM | Trichoderma viride | |
| 3.2.1.37 | Hg2+ | strong inhibition at 0.1 mM | Trichoderma viride | |
| 3.2.1.37 | methyl-beta-D-xylopyranoside | 34% inhibition at 10 mM | Trichoderma viride | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.37 | 0.08 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Trichoderma viride | |
| 3.2.1.37 | 5 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Trichoderma viride | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.37 | extracellular | - |
Trichoderma viride | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.37 | Fe2+ | stimulation at 1 mM | Trichoderma viride | |
| 3.2.1.37 | Mn2+ | stimulation at 1 mM | Trichoderma viride | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 50000 | - |
2 * 50000, SDS-PAGE | Trichoderma viride |
| 3.2.1.37 | 100000 | - |
gel filtration | Trichoderma viride |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.37 | xylan + H2O | Trichoderma viride | limited capability to produce xylose due to synergistic action of xylanase and beta-xylosidase, synergistic action also needed for libration of acidic substituents | beta-D-xylose + ? | - |
? | |
| 3.2.1.37 | xylo-oligosaccharides + H2O | Trichoderma viride | smaller xylooligosaccharides from 2-5mers less rapidly hydrolyzed | beta-D-xylose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.8 | Trichoderma viride | - |
- |
- |
| 3.2.1.37 | Trichoderma viride | - |
three isoforms | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | glycoprotein | 25% carbohydrate moiety | Trichoderma viride |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.8 | xylanase A and B | Trichoderma viride |
| 3.2.1.37 | to homogeneity by 2step chromatography | Trichoderma viride |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.37 | cell culture | - |
Trichoderma viride | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 2.4 | - |
- |
Trichoderma viride |
| 3.2.1.37 | 28.2 | - |
- |
Trichoderma viride |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.8 | 1,4-beta-D-xylan + H2O | - |
Trichoderma viride | additional information | - |
? | |
| 3.2.1.37 | p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma viride | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.37 | xylan + H2O | limited capability to produce xylose due to synergistic action of xylanase and beta-xylosidase, synergistic action also needed for libration of acidic substituents | Trichoderma viride | beta-D-xylose + ? | - |
? | |
| 3.2.1.37 | xylo-oligosaccharides + H2O | smaller xylooligosaccharides from 2-5mers less rapidly hydrolyzed | Trichoderma viride | beta-D-xylose + ? | - |
? | |
| 3.2.1.37 | xyloheptaose + H2O | - |
Trichoderma viride | beta-D-xylose + ? | - |
? | |
| 3.2.1.37 | xylohexaose + H2O | - |
Trichoderma viride | beta-D-xylose + ? | - |
? | |
| 3.2.1.37 | xylooctaose + H2O | - |
Trichoderma viride | beta-D-xylose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | dimer | 2 * 50000, SDS-PAGE | Trichoderma viride |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | beta-D-xyloside xylohydrolase | - |
Trichoderma viride |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 70 | - |
- |
Trichoderma viride |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 70 | - |
70% activity after 1 h | Trichoderma viride |
| 3.2.1.37 | 80 | - |
rapidly inactivated above | Trichoderma viride |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 4.5 | - |
- |
Trichoderma viride |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 3.5 | 7.5 | - |
Trichoderma viride |
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