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Literature summary extracted from

  • Dobberstein, J.; Emeis, C.C.
    Purification and characterization of beta-xylosidase from Aureobasidium pullulans (1991), Appl. Microbiol. Biotechnol., 35, 210-215.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.37 Acetate buffer increasing activity Aureobasidium pullulans
3.2.1.37 dithiothreitol increasing activity at 100 mM Aureobasidium pullulans

General Stability

EC Number General Stability Organism
3.2.1.37 citrate phosphate and piperazine-HCl buffers reduce activity Aureobasidium pullulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 EDTA complete inactivation at 100 mM Aureobasidium pullulans
3.2.1.37 SDS slight inhibition at 0.1%, w/v Aureobasidium pullulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.43
-
 p-nitrophenyl beta-D-xyloside
-
 Aureobasidium pullulans
3.2.1.37 17.5
-
 xylobiose
-
 Aureobasidium pullulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Aureobasidium pullulans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 121000
-
 2 * 121000, SDS-PAGE Aureobasidium pullulans
3.2.1.37 224000
-
 gel filtration, SDS-PAGE Aureobasidium pullulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 xylobiose + H2O Aureobasidium pullulans
-
 beta-D-xylose + D-xylose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Aureobasidium pullulans
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein N-glycosylated with high mannose structure Aureobasidium pullulans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 to homogeneity by 4step chromatography techniques Aureobasidium pullulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 mycelium highest enzyme production in xylan medium Aureobasidium pullulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 7.3
-
-
 Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 p-nitrophenyl beta-D-xyloside + H2O
-
 Aureobasidium pullulans p-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 xylobiose + H2O
-
 Aureobasidium pullulans beta-D-xylose + D-xylose
-
 ?
3.2.1.37 xylotriose + H2O also transxylosylation activity to xylotetraose Aureobasidium pullulans beta-D-xylose + xylobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 dimer 2 * 121000, SDS-PAGE Aureobasidium pullulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 80
-
-
 Aureobasidium pullulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 70
-
 27% loss of activity after 60 min Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 4.5
-
-
 Aureobasidium pullulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 2 9.5 stable within Aureobasidium pullulans