BRENDA - Enzyme Database

Genetic organization, sequence and biochemical characterzation of recombinant beta-xylosidase from Thermoanaerobacterium saccharolyticumm strain B6A-RI

Lee, Y.E.; Zeikus, J.G.; J. Gen. Microbiol. 139, 1235-1243 (1993)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.37
expression in Escherichia coli
Thermoanaerobacterium saccharolyticum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.37
55000
-
SDS-PAGE, calculation from sequence of cDNA
Thermoanaerobacterium saccharolyticum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.37
xylo-oligosaccharides + H2O
Thermoanaerobacterium saccharolyticum
-
beta-D-xylose + ?
-
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylobiose + H2O
Thermoanaerobacterium saccharolyticum
-
beta-D-xylose + D-xylose
-
Thermoanaerobacterium saccharolyticum
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.37
Thermoanaerobacterium saccharolyticum
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.37
to homogeneity by heat treatment and 2step chromatography techniques
Thermoanaerobacterium saccharolyticum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.37
5.53
-
-
Thermoanaerobacterium saccharolyticum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.37
o-nitrophenyl beta-D-xylopyranoside + H2O
-
136280
Thermoanaerobacterium saccharolyticum
o-nitrophenol + beta-D-xylopyranose
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
p-nitrophenyl beta-D-xylopyranoside + H2O
high specificity, transxylosylation activity
136280
Thermoanaerobacterium saccharolyticum
p-nitrophenol + beta-D-xylopyranose
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylo-oligosaccharides + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose + ?
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylobiose + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose + D-xylose
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylopentaose + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose + ?
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylotetraose + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylotriose + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose + xylobiose
-
136280
Thermoanaerobacterium saccharolyticum
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.37
monomer
1 * 55000, SDS-PAGE
Thermoanaerobacterium saccharolyticum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
70
-
-
Thermoanaerobacterium saccharolyticum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
65
-
stable up to, loss of activity at 85°C
Thermoanaerobacterium saccharolyticum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
5.5
-
-
Thermoanaerobacterium saccharolyticum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.37
5
6.6
high activity within
Thermoanaerobacterium saccharolyticum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.37
expression in Escherichia coli
Thermoanaerobacterium saccharolyticum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.37
55000
-
SDS-PAGE, calculation from sequence of cDNA
Thermoanaerobacterium saccharolyticum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.37
xylo-oligosaccharides + H2O
Thermoanaerobacterium saccharolyticum
-
beta-D-xylose + ?
-
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylobiose + H2O
Thermoanaerobacterium saccharolyticum
-
beta-D-xylose + D-xylose
-
Thermoanaerobacterium saccharolyticum
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.37
to homogeneity by heat treatment and 2step chromatography techniques
Thermoanaerobacterium saccharolyticum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.37
5.53
-
-
Thermoanaerobacterium saccharolyticum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.37
o-nitrophenyl beta-D-xylopyranoside + H2O
-
136280
Thermoanaerobacterium saccharolyticum
o-nitrophenol + beta-D-xylopyranose
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
p-nitrophenyl beta-D-xylopyranoside + H2O
high specificity, transxylosylation activity
136280
Thermoanaerobacterium saccharolyticum
p-nitrophenol + beta-D-xylopyranose
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylo-oligosaccharides + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose + ?
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylobiose + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose + D-xylose
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylopentaose + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose + ?
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylotetraose + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose
-
136280
Thermoanaerobacterium saccharolyticum
?
3.2.1.37
xylotriose + H2O
-
136280
Thermoanaerobacterium saccharolyticum
beta-D-xylose + xylobiose
-
136280
Thermoanaerobacterium saccharolyticum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.37
monomer
1 * 55000, SDS-PAGE
Thermoanaerobacterium saccharolyticum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
70
-
-
Thermoanaerobacterium saccharolyticum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
65
-
stable up to, loss of activity at 85°C
Thermoanaerobacterium saccharolyticum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
5.5
-
-
Thermoanaerobacterium saccharolyticum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.37
5
6.6
high activity within
Thermoanaerobacterium saccharolyticum