Any feedback?
Literature summary extracted from
- Genetic organization, sequence and biochemical characterzation of recombinant beta-xylosidase from Thermoanaerobacterium saccharolyticumm strain B6A-RI (1993), J. Gen. Microbiol., 139, 1235-1243.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.37 | expression in Escherichia coli | Thermoanaerobacterium saccharolyticum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 55000 | - |
SDS-PAGE, calculation from sequence of cDNA | Thermoanaerobacterium saccharolyticum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.37 | xylo-oligosaccharides + H2O | Thermoanaerobacterium saccharolyticum | - |
beta-D-xylose + ? | - |
? |  |
| 3.2.1.37 | xylobiose + H2O | Thermoanaerobacterium saccharolyticum | - |
beta-D-xylose + D-xylose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.37 | Thermoanaerobacterium saccharolyticum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.37 | to homogeneity by heat treatment and 2step chromatography techniques | Thermoanaerobacterium saccharolyticum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 5.53 | - |
- |
Thermoanaerobacterium saccharolyticum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.37 | o-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermoanaerobacterium saccharolyticum | o-nitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.37 | p-nitrophenyl beta-D-xylopyranoside + H2O | high specificity, transxylosylation activity | Thermoanaerobacterium saccharolyticum | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.37 | xylo-oligosaccharides + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + ? | - |
? | |
| 3.2.1.37 | xylobiose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + D-xylose | - |
? | |
| 3.2.1.37 | xylopentaose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + ? | - |
? | |
| 3.2.1.37 | xylotetraose + H2O | - |
Thermoanaerobacterium saccharolyticum | xylotriose + beta-D-xylose | - |
? | |
| 3.2.1.37 | xylotriose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + xylobiose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | monomer | 1 * 55000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 70 | - |
- |
Thermoanaerobacterium saccharolyticum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 65 | - |
stable up to, loss of activity at 85°C | Thermoanaerobacterium saccharolyticum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 5.5 | - |
- |
Thermoanaerobacterium saccharolyticum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 5 | 6.6 | high activity within | Thermoanaerobacterium saccharolyticum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
