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Literature summary extracted from

  • Ali, S.; Malek, S.; Hossain, Z.
    Purification and characterization of a thermostable glucoamylase from a Myrothecium isolate (1994), J. Appl. Bacteriol., 76, 210-215.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Hg2+ potent inhibitor for glucoamylase I and II Myrothecium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
additional information
-
Myrothecium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 72000
-
x * 72000, glucoamylase I, SDS-PAGE Myrothecium sp.
3.2.1.3 96000
-
x * 96000, glucoamylase II, SDS-PAGE Myrothecium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Myrothecium sp.
-
-
-
3.2.1.3 Myrothecium sp. M1
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein glucoamylase I and II Myrothecium sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 glucoamylase I and II Myrothecium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 culture filtrate
-
Myrothecium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 250
-
-
Myrothecium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 amylose + H2O
-
Myrothecium sp. glucose + ?
-
?
3.2.1.3 amylose + H2O
-
Myrothecium sp. M1 glucose + ?
-
?
3.2.1.3 maltose + H2O
-
Myrothecium sp. 2 glucose
-
?
3.2.1.3 maltose + H2O
-
Myrothecium sp. M1 2 glucose
-
?
3.2.1.3 maltotriose + H2O
-
Myrothecium sp. maltose + glucose
-
?
3.2.1.3 maltotriose + H2O
-
Myrothecium sp. M1 maltose + glucose
-
?
3.2.1.3 starch + H2O
-
Myrothecium sp. glucose + ?
-
?
3.2.1.3 starch + H2O
-
Myrothecium sp. M1 glucose + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 72000, glucoamylase I, SDS-PAGE Myrothecium sp.
3.2.1.3 ? x * 96000, glucoamylase II, SDS-PAGE Myrothecium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 70
-
glucoamylase I and II Myrothecium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 40 70 30 min, glucoamylase I and II, stable Myrothecium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4
-
glucoamylase I and II Myrothecium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 3 6 glucoamylase I and II, stable Myrothecium sp.