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Literature summary extracted from

  • Brandani da Silva, W.; Peralta, R.M.
    Purification and characterization of a thermostable glucoamylase from Aspergillus fumigatus (1998), Can. J. Microbiol., 44, 493-497.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Hg2+ 10 mM, strong inhibition Aspergillus fumigatus
3.2.1.3 additional information remarkable insensitivity of the enzyme to end product inhibition Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 42000
-
 gel filtration Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus fumigatus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein contains 23% carbohydrate Aspergillus fumigatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3
-
 Aspergillus fumigatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 150
-
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 alpha-cyclodextrin + H2O no activity Aspergillus fumigatus glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O 85% of activity with starch Aspergillus fumigatus D-glucose + ?
-
 ?
3.2.1.3 amylose + H2O 93% of the activity with starch Aspergillus fumigatus glucose + ?
-
 ?
3.2.1.3 glycogen + H2O 82% of the activity with starch Aspergillus fumigatus glucose + ?
-
 ?
3.2.1.3 isomaltose + H2O 10% of the activity with starch Aspergillus fumigatus glucose
-
 ?
3.2.1.3 maltose + H2O 40% of the activity with starch Aspergillus fumigatus 2 glucose
-
 ?
3.2.1.3 maltotriose + H2O 46% of the activity with starch Aspergillus fumigatus maltose + glucose
-
 ?
3.2.1.3 pullulan + H2O 52% of the activity with starch Aspergillus fumigatus ?
-
 ?
3.2.1.3 starch + H2O soluble Aspergillus fumigatus glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 monomer 1 * 42000, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 65
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 70
-
 half-life in absence of starch: 15 min. Half-life in presene of 3% w/v starch or sorbitol: 50 min. Half-life in presence of 10% sorbitol: 40 min. Half-life in presence of 30% sorbitol: 65 min Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4 4.5
-
 Aspergillus fumigatus
3.2.1.3 4.5 5.5
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 3 7 40°C, 2 days, stable Aspergillus fumigatus