EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.3 | S411A | 54-74% of the catalytic efficiency of the wild type enzyme. Increased pH-optimum by 0.8 units for both maltose and maltoheptaose hydrolysis while maintaining a high level of activity and catalytic efficiency. In hydrolysis of 28% DE 10 maltodextrin, the mutant enzyme has a pH optimum of 7 compared with 5.6 for wild-type enzyme, and has higher initial rates of glucose production than wild-type enzyme at all pH values tested above pH 6.6 | Aspergillus awamori |
3.2.1.3 | S411C | 54-74% of the catalytic efficiency of the wild type enzyme | Aspergillus awamori |
3.2.1.3 | S411D | 6-12% of the catalytic efficiency of the wild type enzyme | Aspergillus awamori |
3.2.1.3 | S411G | catalytic efficiency like that of wild type enzyme for isomaltose, maltose and maltoheptaose hydrolysis at pH 4.4 | Aspergillus awamori |
3.2.1.3 | S411H | 6-12% of the catalytic efficiency of the wild type enzyme | Aspergillus awamori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | 0.07 | - |
maltoheptaose | mutant enzyme S411C | Aspergillus awamori | |
3.2.1.3 | 0.083 | - |
maltoheptaose | wild-type enzyme | Aspergillus awamori | |
3.2.1.3 | 0.104 | - |
maltoheptaose | mutant enzyme S411A | Aspergillus awamori | |
3.2.1.3 | 0.132 | - |
maltoheptaose | mutant enzyme S411G | Aspergillus awamori | |
3.2.1.3 | 0.148 | - |
maltoheptaose | mutant enzyme S411D | Aspergillus awamori | |
3.2.1.3 | 0.53 | - |
maltose | mutant enzyme S411C | Aspergillus awamori | |
3.2.1.3 | 1.01 | - |
maltose | wild-type enzyme | Aspergillus awamori | |
3.2.1.3 | 1.26 | - |
maltose | mutant enzyme S411A | Aspergillus awamori | |
3.2.1.3 | 1.59 | - |
maltose | mutant enzyme S411G | Aspergillus awamori | |
3.2.1.3 | 3.58 | - |
maltose | mutant enzyme S411D | Aspergillus awamori | |
3.2.1.3 | 12.3 | - |
isomaltose | mutant enzyme S411C | Aspergillus awamori | |
3.2.1.3 | 23.5 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
3.2.1.3 | 26.2 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
3.2.1.3 | 27.9 | - |
isomaltose | mutant enzyme S411A | Aspergillus awamori |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Aspergillus awamori | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | isomaltose + H2O | - |
Aspergillus awamori | glucose | - |
? | |
3.2.1.3 | maltoheptaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
3.2.1.3 | maltose + H2O | - |
Aspergillus awamori | 2 glucose | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | 0.22 | - |
isomaltose | mutant enzyme S411C | Aspergillus awamori | |
3.2.1.3 | 0.63 | - |
isomaltose | mutant enzyme S411A | Aspergillus awamori | |
3.2.1.3 | 0.72 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
3.2.1.3 | 0.93 | - |
isomaltose | mutant enzyme S411G | Aspergillus awamori | |
3.2.1.3 | 4.36 | - |
maltose | mutant enzyme S411D | Aspergillus awamori | |
3.2.1.3 | 7.78 | - |
maltose | mutant enzyme S411C | Aspergillus awamori | |
3.2.1.3 | 14.5 | - |
maltose | mutant enzyme S411G | Aspergillus awamori | |
3.2.1.3 | 15.8 | - |
maltoheptaose | mutant enzyme S411D | Aspergillus awamori | |
3.2.1.3 | 18.9 | - |
maltose | mutant enzyme S411A | Aspergillus awamori | |
3.2.1.3 | 20.4 | - |
maltose | wild-type enzyme | Aspergillus awamori | |
3.2.1.3 | 33 | - |
maltoheptaose | mutant enzyme S411C | Aspergillus awamori | |
3.2.1.3 | 59.4 | - |
maltoheptaose | mutant enzyme S411A | Aspergillus awamori | |
3.2.1.3 | 72.3 | - |
maltoheptaose | wild-type enzyme | Aspergillus awamori | |
3.2.1.3 | 84 | - |
maltoheptaose | mutant enzyme S411G | Aspergillus awamori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 4 | - |
hydrolysis of maltose, wild-type enzyme | Aspergillus awamori |
3.2.1.3 | 4.1 | - |
hydrolysis of maltose, mutant enzyme S411H | Aspergillus awamori |
3.2.1.3 | 4.2 | - |
hydrolysis of maltose, mutant enzyme S411G | Aspergillus awamori |
3.2.1.3 | 4.4 | - |
hydrolysis of maltose, mutant enzyme S411D | Aspergillus awamori |
3.2.1.3 | 4.7 | - |
hydrolysis of maltoheptaose, wild-type enzyme | Aspergillus awamori |
3.2.1.3 | 4.8 | - |
hydrolysis of maltose, mutant enzyme S411A and S411C | Aspergillus awamori |
3.2.1.3 | 5 | - |
hydrolysis of maltoheptaose, mutant enzyme S411G | Aspergillus awamori |
3.2.1.3 | 5.5 | - |
hydrolysis of maltoheptaose, mutant enzyme S411A | Aspergillus awamori |