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Literature summary extracted from

  • Takesue, Y.; Takesue, S.
    Purification and characterization of alpha-glucosidase complex from the intestine of the frog, Rana japonica (1996), Biochim. Biophys. Acta, 1296, 152-158.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 0.7
-
 amylopectin
-
 Rana japonica
3.2.1.3 1.1
-
 maltose
-
 Rana japonica
3.2.1.3 1.4
-
 glycogen
-
 Rana japonica
3.2.1.3 1.7
-
 starch soluble Rana japonica
3.2.1.3 1.8
-
 amylose
-
 Rana japonica
3.2.1.3 4
-
 isomaltose
-
 Rana japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 116000
-
 1 * 116000 + 1 * 212000, SDS-PAGE Rana japonica
3.2.1.3 212000
-
 1 * 116000 + 1 * 212000, SDS-PAGE Rana japonica
3.2.1.3 440000
-
 papain solubilized enzyme, gel filtration Rana japonica
3.2.1.3 550000
-
 detergent solubilized enzyme, gel filtration Rana japonica
3.2.1.20 116000
-
 1 * 116000 + 1 * 212000, SDS-PAGE Rana japonica
3.2.1.20 212000
-
 1 * 116000 + 1 * 212000, SDS-PAGE Rana japonica
3.2.1.20 550000
-
 gel filtration Rana japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 additional information Rana japonica the enzyme is responsible for much of the isomaltase and maltase activities in the intestine of the frog ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Rana japonica
-
-
-
3.2.1.20 Rana japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3
-
 Rana japonica
3.2.1.20
-
 Rana japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 intestine brush border membrane Rana japonica
-
3.2.1.20 intestine brush-border membrane Rana japonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 amylopectin + H2O
-
 Rana japonica D-glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Rana japonica glucose + ?
-
 ?
3.2.1.3 glycogen + H2O
-
 Rana japonica glucose + ?
-
 ?
3.2.1.3 isomaltose + H2O
-
 Rana japonica glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Rana japonica 2 glucose
-
 ?
3.2.1.3 additional information the enzyme is responsible for much of the isomaltase and maltase activities in the intestine of the frog Rana japonica ?
-
 ?
3.2.1.3 starch + H2O soluble Rana japonica glucose + ?
-
 ?
3.2.1.20 amylopectin + H2O
-
 Rana japonica alpha-D-glucose
-
 ?
3.2.1.20 amylose + H2O
-
 Rana japonica alpha-D-glucose
-
 ?
3.2.1.20 glycogen + H2O
-
 Rana japonica alpha-D-glucose
-
 ?
3.2.1.20 isomaltose + H2O
-
 Rana japonica 2 alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Rana japonica alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 starch + H2O soluble Rana japonica alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 dimer 1 * 116000 + 1 * 212000, SDS-PAGE Rana japonica
3.2.1.20 dimer 1 * 116000 + 1 * 212000, SDS-PAGE Rana japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 6 7 hydrolysis of isomaltose Rana japonica
3.2.1.3 6.5 7.5 hydrolysis of soluble starch Rana japonica
3.2.1.3 7 8 hydrolysis of maltose Rana japonica
3.2.1.20 6 7 hydrolysis of isomaltose Rana japonica
3.2.1.20 6.5 7.5 hydrolysis of soluble starch Rana japonica
3.2.1.20 7 8 hydrolysis of maltose Rana japonica