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Literature summary extracted from

  • Yamasaki, Y.; Suzuki, Y.; Ozawa, J.
    Purification and properties of two forms of glucoamylase from Penicillium oxalicum (1977), Agric. Biol. Chem., 41, 755-762.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 84000
-
glucoamylase I, calculation from diffusion and sedimentation data Penicillium oxalicum
3.2.1.3 86000
-
glucoamylase II, calculation from diffusion and sedimentation data Penicillium oxalicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Penicillium oxalicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 glucoamylase I and II Penicillium oxalicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 377.4
-
glucoamylase I Penicillium oxalicum
3.2.1.3 457.5
-
glucoamylase II Penicillium oxalicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O soluble Penicillium oxalicum glucose + ?
-
?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 45 70 45°C: about 35% of maximal activity, glucoamylase I, about 60% of maximal activity, glucoamylase II. 70°C: about 30% of maximal activity, glucoamylase I and II Penicillium oxalicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 55
-
15 min, stable up to Penicillium oxalicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
glucoamylase II Penicillium oxalicum
3.2.1.3 5
-
glucoamylase I Penicillium oxalicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 3 6 pH 3.0: about 40% of maximal activity for glucoamylase II, about 70% of maximal activity for glucoamylase I. pH 6.0: about 75% of maximal activity for glucoamylase I and II Penicillium oxalicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 3 6.5 30°C, 20 h, stable Penicillium oxalicum