EC Number | General Stability | Organism |
---|---|---|
3.2.1.3 | 5 M guanidine hydrochloride, almost complete denaturation | Aspergillus phoenicis |
3.2.1.3 | 8 M urea, partial denaturation | Aspergillus phoenicis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.3 | Hg2+ | 1 mM, 48% inhibition, glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | Mn2+ | 1 mM, 12% inhibition, glucoamylase M2 | Aspergillus phoenicis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | additional information | - |
additional information | 0.92% for soluble starch, glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 0.146 | - |
amylose | glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 0.73 | - |
Dextrin | glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 1.25 | - |
glycogen | glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 1.4 | - |
maltotriose | glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 1.65 | - |
amylopectin | glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 3 | - |
maltose | glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 5.6 | - |
p-nitrophenyl-alpha-D-glucoside | glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 10 | - |
Phenyl-alpha-maltoside | glucoamylase M2 | Aspergillus phoenicis | |
3.2.1.3 | 36.4 | - |
isomaltose | glucoamylase M2 | Aspergillus phoenicis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 70000 | - |
x * 70000, glucoamylase M2, SDS-PAGE | Aspergillus phoenicis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Aspergillus phoenicis | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.3 | glycoprotein | contains 0.55% glucosamine and 12% neutral sugar which consists of a large amount of mannose and small amounts of galactose and glucose | Aspergillus phoenicis |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.3 | glucoamylase M2 | Aspergillus phoenicis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 32.6 | - |
glucoamylase M1 | Aspergillus phoenicis |
3.2.1.3 | 40.8 | - |
glucoamylase M2 | Aspergillus phoenicis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | 4-nitrophenyl alpha-D-glucoside + H2O | - |
Aspergillus phoenicis | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.3 | amylopectin + H2O | - |
Aspergillus phoenicis | D-glucose + ? | - |
? | |
3.2.1.3 | amylose + H2O | - |
Aspergillus phoenicis | glucose + ? | - |
? | |
3.2.1.3 | dextrin + H2O | - |
Aspergillus phoenicis | ? | - |
? | |
3.2.1.3 | glycogen + H2O | - |
Aspergillus phoenicis | glucose + ? | - |
? | |
3.2.1.3 | isomaltose + H2O | - |
Aspergillus phoenicis | glucose | - |
? | |
3.2.1.3 | maltose + H2O | - |
Aspergillus phoenicis | 2 glucose | - |
? | |
3.2.1.3 | maltotriose + H2O | - |
Aspergillus phoenicis | maltose + glucose | - |
? | |
3.2.1.3 | phenyl alpha-maltoside + H2O | - |
Aspergillus phoenicis | glucose + phenyl alpha-glucoside | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.3 | ? | x * 70000, glucoamylase M2, SDS-PAGE | Aspergillus phoenicis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 50 | - |
pH 6.0, 5 min, stable up to | Aspergillus phoenicis |
3.2.1.3 | 70 | - |
pH 6.0, 5 min, complete loss of activity | Aspergillus phoenicis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 4.5 | - |
glucoamylase M2 | Aspergillus phoenicis |
3.2.1.3 | 4.5 | - |
hydrolysis of starch | Aspergillus phoenicis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 2 | - |
37°C, 1 h, complete loss of activity below | Aspergillus phoenicis |
3.2.1.3 | 2.5 | 7.5 | 37°C, 1 h, stable | Aspergillus phoenicis |
3.2.1.3 | 9 | - |
37°C, 1 h, complete loss of activity above | Aspergillus phoenicis |