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Literature summary extracted from
- Purification and substrate specificity of glucoamylase of Paecilomyces varioti AHU 9417 (1985), Agric. Biol. Chem., 49, 1633-1641.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.3 | 0.0011 | - |
soluble starch | soluble | Paecilomyces variotii | |
| 3.2.1.3 | 0.03 | - |
amylose | DP = 17 | Paecilomyces variotii |  |
| 3.2.1.3 | 0.04 | - |
amylose | DP = 13 | Paecilomyces variotii | |
| 3.2.1.3 | 0.11 | - |
maltoheptaose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 0.12 | - |
maltohexaose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 0.14 | - |
maltotetraose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 0.26 | - |
maltotriose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 0.31 | - |
Phenyl alpha-maltoside | - |
Paecilomyces variotii | |
| 3.2.1.3 | 1.2 | - |
maltose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 12 | - |
panose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 22 | - |
nigerose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 59 | - |
isomaltose | - |
Paecilomyces variotii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 69000 | - |
gel filtration | Paecilomyces variotii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | Paecilomyces variotii | - |
AHU 9417 | - |
| 3.2.1.3 | Paecilomyces variotii AHU 9417 | - |
AHU 9417 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.3 | - |
Paecilomyces variotii |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | culture medium | - |
Paecilomyces variotii | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | additional information | - |
- |
Paecilomyces variotii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | amylopectin + H2O | - |
Paecilomyces variotii | D-glucose + ? | - |
? | |
| 3.2.1.3 | amylopectin + H2O | - |
Paecilomyces variotii AHU 9417 | D-glucose + ? | - |
? | |
| 3.2.1.3 | amylose + H2O | - |
Paecilomyces variotii | glucose + ? | - |
? | |
| 3.2.1.3 | amylose + H2O | - |
Paecilomyces variotii AHU 9417 | glucose + ? | - |
? | |
| 3.2.1.3 | dextrin + H2O | beta-limit dextrin | Paecilomyces variotii | ? | - |
? | |
| 3.2.1.3 | dextrin + H2O | beta-limit dextrin | Paecilomyces variotii AHU 9417 | ? | - |
? | |
| 3.2.1.3 | glycogen + H2O | - |
Paecilomyces variotii | glucose + ? | - |
? | |
| 3.2.1.3 | glycogen + H2O | - |
Paecilomyces variotii AHU 9417 | glucose + ? | - |
? | |
| 3.2.1.3 | isomaltose + H2O | - |
Paecilomyces variotii | glucose | - |
? | |
| 3.2.1.3 | maltoheptaose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| 3.2.1.3 | maltohexaose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| 3.2.1.3 | maltopentaose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| 3.2.1.3 | maltose + H2O | - |
Paecilomyces variotii | 2 glucose | - |
? | |
| 3.2.1.3 | maltotetraose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| 3.2.1.3 | maltotriose + H2O | - |
Paecilomyces variotii | maltose + glucose | - |
? | |
| 3.2.1.3 | nigerose + H2O | - |
Paecilomyces variotii | 2 alpha-D-glucose | - |
? | |
| 3.2.1.3 | panose + H2O | - |
Paecilomyces variotii | ? | - |
? | |
| 3.2.1.3 | phenyl alpha-maltoside + H2O | - |
Paecilomyces variotii | glucose + phenyl alpha-glucoside | - |
? | |
| 3.2.1.3 | soluble starch + H2O | - |
Paecilomyces variotii | beta-D-glucose + ? | - |
? | |
| 3.2.1.3 | soluble starch + H2O | - |
Paecilomyces variotii | beta-D-glucose + soluble starch | - |
? | |
| 3.2.1.3 | starch + H2O | soluble | Paecilomyces variotii | glucose + ? | beta-glucose | ? | |
| 3.2.1.3 | starch + H2O | soluble | Paecilomyces variotii AHU 9417 | glucose + ? | beta-glucose | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | monomer | 1 * 69000, SDS-PAGE | Paecilomyces variotii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 50 | - |
pH 6.4, 15 min, stable up to | Paecilomyces variotii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.3 | 0.4 | - |
isomaltose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 2.2 | - |
nigerose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 7.2 | - |
maltose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 9.1 | - |
panose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 11 | - |
Phenyl alpha-maltoside | - |
Paecilomyces variotii | |
| 3.2.1.3 | 27.2 | - |
maltotriose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 36.5 | - |
amylose | DP = 17 | Paecilomyces variotii | |
| 3.2.1.3 | 37.7 | - |
amylose | DP = 13 | Paecilomyces variotii | |
| 3.2.1.3 | 41.1 | - |
maltopentaose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 41.6 | - |
maltohexaose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 43.4 | - |
maltotetraose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 46.6 | - |
maltoheptaose | - |
Paecilomyces variotii | |
| 3.2.1.3 | 49.1 | - |
soluble starch | - |
Paecilomyces variotii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 4.5 | - |
- |
Paecilomyces variotii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 4.5 | 10 | 4°C, 24 h, stable | Paecilomyces variotii |
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