Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Buettner, R.; Bode, R.; Birnbaum, D.
    Purification and characterization of the extracellular glucoamylase from the yeast Trichosporon adeninovorans (1987), J. Basic Microbiol., 27, 299-308.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 cellobiose 5 mM, slight inhibition of starch hydrolysis Blastobotrys adeninivorans
3.2.1.3 fructose 5 mM, slight inhibition of starch hydrolysis Blastobotrys adeninivorans
3.2.1.3 sorbitol 5 mM, slight inhibition of starch hydrolysis Blastobotrys adeninivorans
3.2.1.3 xylose 5 mM, slight inhibition of starch hydrolysis Blastobotrys adeninivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information 4 g/l for maltose Blastobotrys adeninivorans
3.2.1.3 additional information
-
 additional information 1.2 g/l for soluble starch Blastobotrys adeninivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
 Blastobotrys adeninivorans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 225000
-
 gel filtration Blastobotrys adeninivorans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Blastobotrys adeninivorans
-
 LS3
-
3.2.1.3 Blastobotrys adeninivorans LS3
-
 LS3
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein
-
 Blastobotrys adeninivorans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3
-
 Blastobotrys adeninivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 additional information
-
-
 Blastobotrys adeninivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 glycogen + H2O
-
 Blastobotrys adeninivorans glucose + ?
-
 ?
3.2.1.3 glycogen + H2O
-
 Blastobotrys adeninivorans LS3 glucose + ?
-
 ?
3.2.1.3 maltose + H2O
-
 Blastobotrys adeninivorans 2 glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Blastobotrys adeninivorans LS3 2 glucose
-
 ?
3.2.1.3 starch + H2O soluble Blastobotrys adeninivorans glucose + ?
-
 ?
3.2.1.3 starch + H2O wheat starch, potato starch, corn starch Blastobotrys adeninivorans glucose + ?
-
 ?
3.2.1.3 starch + H2O soluble Blastobotrys adeninivorans LS3 glucose + ?
-
 ?
3.2.1.3 starch + H2O wheat starch, potato starch, corn starch Blastobotrys adeninivorans LS3 glucose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 60 70 in dependence of substrate concentration Blastobotrys adeninivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 50
-
 15 min, stable in presence of 1.25% soluble starch, about 80% loss of activity without starch Blastobotrys adeninivorans
3.2.1.3 70
-
 fairly stable at approximately 4% of soluble starch Blastobotrys adeninivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4 5.5
-
 Blastobotrys adeninivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 3.5 6 pH 3.5: about 50% of maximal activity, pH 6.0: about 35% of maximal activity Blastobotrys adeninivorans