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Literature summary extracted from

  • Horlacher, R.; Uhland, K.; Klein, W.; Ehrmann, M.; Boos, W.
    Characterization of a cytoplasmic trehalase of Escherichia coli (1996), J. Bacteriol., 178, 6250-6257.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 K+ 0.5 M KCl, activity is 2fold lower Escherichia coli
3.2.1.28 Na+ 0.5 M NaCl, activity is 2fold lower Escherichia coli
3.2.1.28 potassium glutamate 0.5 M KCl, activity is 2fold lower Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 1.9
-
 trehalose cytoplasmic enzyme Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 cytoplasm
-
 Escherichia coli 5737
-
3.2.1.28 periplasm at high osmolarity a periplasmic trehalase is induced Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 additional information cytoplasmic enzyme has no metal dependency Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 64000
-
 1 * 64000, cytoplasmic enzyme, SDS-PAGE Escherichia coli
3.2.1.28 74000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 trehalose + H2O Escherichia coli pathway of trehalose utilization D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 from an overexpressing strain, cytoplasmic enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Escherichia coli D-glucose
-
 ?
3.2.1.28 trehalose + H2O pathway of trehalose utilization Escherichia coli D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 monomer 1 * 64000, cytoplasmic enzyme, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 6
-
-
 Escherichia coli