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Literature summary extracted from

  • Zimmermann, A.L.S.; Terenzi, H.F.; Jorge, J.A.
    Purification and properties of an extracellular conidial trehalase from Humicola grisea var. thermoidea (1990), Biochim. Biophys. Acta, 1036, 41-46.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 ADP Ca2+ abolishes inhibitory effect Trichocladium griseum
3.2.1.28 AMP Ca2+ abolishes inhibitory effect Trichocladium griseum
3.2.1.28 ATP
-
 Trichocladium griseum
3.2.1.28 phosphate Ca2+ abolishes inhibitory effect Trichocladium griseum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 2.3
-
 trehalose
-
 Trichocladium griseum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 extracellular
-
 Trichocladium griseum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Ca2+ activates Trichocladium griseum
3.2.1.28 Co2+ activates Trichocladium griseum
3.2.1.28 Mn2+ activates Trichocladium griseum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 580000
-
 gel filtration Trichocladium griseum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 trehalose + H2O Trichocladium griseum
-
 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Trichocladium griseum
-
 var. thermoidea
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein contains 56% carbohydrate Trichocladium griseum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28
-
 Trichocladium griseum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 conidium
-
 Trichocladium griseum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 73.9
-
-
 Trichocladium griseum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Trichocladium griseum D-glucose
-
 ?
3.2.1.28 trehalose + H2O absolute specificity Trichocladium griseum D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 More polypeptide bands of 84000 Da, 98000 Da and 105000 Da are detected on SDS-PAGE Trichocladium griseum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 60
-
-
 Trichocladium griseum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.28 40 75 about 35% of maximal activity at 40°C and at 75°C Trichocladium griseum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 5.6
-
 sodium acetate buffer Trichocladium griseum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.28 4 7.5 pH 4.0: about 35% of maximal activity, pH 7.5: about 30% of maximal activity Trichocladium griseum