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Literature summary extracted from
- Characterization of an acid trehalase of Saccharomyces cerevisiae present in trehalase-sucrase aggregate (1996), Biochim. Biophys. Acta, 1290, 95-100.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.28 | trehalase is stabilized by aggregation with sucrase | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | acetate | - |
Saccharomyces cerevisiae |  |
| 3.2.1.28 | glycerol | 2.5% | Saccharomyces cerevisiae | |
| 3.2.1.28 | phosphate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 5 | - |
trehalose | - |
Saccharomyces cerevisiae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | acid trehalase-sucrase aggregate | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.28 | 0-4°C, pH 4.5, 50% loss of activity after 1 month | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | trehalose + H2O | - |
Saccharomyces cerevisiae | D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 40 | 45 | - |
Saccharomyces cerevisiae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 30 | 50 | 30°C: 80% of maximal activity, 50°C: 50% of maximal activity | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 5 | - |
- |
Saccharomyces cerevisiae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 3.5 | - |
15 min, around 40% loss of activity | Saccharomyces cerevisiae |
| 3.2.1.28 | 7 | - |
15 min, around 40% loss of activity | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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