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Literature summary extracted from

  • Talbot, B.G.; Muir, J.G.; Huber, R.E.
    Properties of a free and a solubilized form of bound alpha,alpha-trehalase purified from honey bee thorax (1975), Can. J. Biochem., 53, 1106-1117.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.28 8 M urea, 30°C, 10 min, 25% loss of activity Apis mellifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 iodoacetamide 100 mM required for inhibition of more than 50% Apis mellifera
3.2.1.28 p-aminophenyl-beta-D-glucoside
-
 Apis mellifera
3.2.1.28 p-nitrophenyl-beta-D-glucoside
-
 Apis mellifera
3.2.1.28 PCMB 1 mM is required for inhibition of more than 50% Apis mellifera
3.2.1.28 phenyl-beta-D-glucoside
-
 Apis mellifera
3.2.1.28 sucrose
-
 Apis mellifera
3.2.1.28 Tris
-
 Apis mellifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.89
-
 trehalose
-
 Apis mellifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 36000
-
 x * 36000, SDS-PAGE Apis mellifera
3.2.1.28 65000
-
 gel filtration Apis mellifera

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Apis mellifera
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 side-chain modification enzyme contains some type of bound lipid Apis mellifera

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28
-
 Apis mellifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 thorax free and bound enzyme form Apis mellifera
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 50
-
 free enzyme Apis mellifera
3.2.1.28 53.4
-
 solubilized enzyme Apis mellifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Apis mellifera D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 ? x * 36000, SDS-PAGE Apis mellifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 3.5
-
 a minor activity maximum at pH 3.5 and a major activity maximum at pH 6.5 Apis mellifera
3.2.1.28 6.5
-
 a minor activity maximum at pH 3.5 and a major activity maximum at pH 6.5 Apis mellifera