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Literature summary extracted from
- Purification and characterization of kidney and intestinal brush-border membrane trehalases from the rabbit (1984), Biochim. Biophys. Acta, 789, 10-19.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | phlorizin | - |
Oryctolagus cuniculus |  |
| 3.2.1.28 | sucrose | competitive | Oryctolagus cuniculus | |
| 3.2.1.28 | Tris | competitive | Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 3.5 | - |
trehalose | pH 6.0 | Oryctolagus cuniculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.28 | brush border | trehalase accounts for 0.1% and 0.3% of total brush-border membrane protein of intestine and kidney, respectively | Oryctolagus cuniculus | 5903 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 75000 | - |
1 * 75000, enzyme from kidney and intestine, SDS-PAGE | Oryctolagus cuniculus |
| 3.2.1.28 | 85000 | - |
enzyme from kidney and intestine, gel filtration | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Oryctolagus cuniculus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | glycoprotein | - |
Oryctolagus cuniculus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | intestine | brush-border membrane | Oryctolagus cuniculus | - |
| 3.2.1.28 | kidney | brush border membrane | Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 312 | - |
kidney | Oryctolagus cuniculus |
| 3.2.1.28 | 343 | - |
intestine | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | trehalose + H2O | - |
Oryctolagus cuniculus | D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | monomer | 1 * 75000, enzyme from kidney and intestine, SDS-PAGE | Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 50 | - |
stable up to | Oryctolagus cuniculus |
| 3.2.1.28 | 70 | - |
3 min, 90% loss of activity | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 5.5 | 6 | - |
Oryctolagus cuniculus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 4.5 | 7 | 37°C, 2 h, stable | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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