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Literature summary extracted from

  • Nakano, M.; Sacktor, B.
    Isolation and characterization of four forms of trehalase from rabbit kidney cortex (1985), J. Biochem., 97, 1329-1335.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.28 enzyme form A1 becomes labile on addition of glycerol, activity in presence of 20% or 40% glycerol is about 30-40% lower than in absence of glycerol. Enzyme form B2 becomes more stable on addition of glycerol, activity in presence of 20% or 40% glycerol is about 30 higher than in absence of glycerol Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 Hg2+ the glycoprotein enzyme forms are more susceptible than the nonglycoprotein forms Oryctolagus cuniculus
3.2.1.28 methyl beta-glucoside
-
 Oryctolagus cuniculus
3.2.1.28 phlorizin
-
 Oryctolagus cuniculus
3.2.1.28 Tris
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Na+ the glycoprotein enzyme forms show lower activation than the nonglycoprotein forms Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 175000
-
 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Oryctolagus cuniculus
-
 2 nonglycoprotein enzyme forms: A1 and B1 and 2 glycoprotein enzyme form A2 and B2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein 2 nonglycoprotein enzyme forms: A1 and B1, and 2 glycoprotein enzyme forms: A2 and B2 Oryctolagus cuniculus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 4 enzyme form Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 kidney cortex Oryctolagus cuniculus
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.28 -80°C, 9 days, 15% loss of activity of enzyme form A1, 5% loss of activity of enzyme form A2, 35% loss of activity of enzyme form B1, 65% loss of activity of enzyme form B2 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Oryctolagus cuniculus D-glucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 5.8 6 phosphate buffer Oryctolagus cuniculus