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Literature summary extracted from

  • Morin, P.R.; Potier, M.
    Intestinal and renal origin of trehalase activity in rabbit amniotic fluid (1987), Biochim. Biophys. Acta, 923, 371-380.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 3.15
-
 trehalose enzyme form kidney, intestine and amniotic fluid Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 brush border
-
 Oryctolagus cuniculus 5903
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 48500
-
 enzyme form amniotic fluid, protein part of the enzyme, radiation inactivation Oryctolagus cuniculus
3.2.1.28 49000
-
 enzyme form kidney and intestine, protein part of the enzyme, radiation inactivation Oryctolagus cuniculus
3.2.1.28 66300 72000 depending on the amount of sugar bound to the enzyme, enzyme from amniotic fluid, calculation from hydrodynamic parameters Oryctolagus cuniculus
3.2.1.28 91000
-
 enzyme from amniotic fluid, gel filtration Oryctolagus cuniculus
3.2.1.28 170000
-
 enzyme from kidney and intestine, gel filtration in presence of 1% Triton Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 amniotic fluid early in gestation amniotic fluid enzyme activity originates exclusively from the fetal kidney, but more and more intestinal enzyme is released into the amniotic cavity as the fetus develops Oryctolagus cuniculus
-
3.2.1.28 intestine brush-border membrane Oryctolagus cuniculus
-
3.2.1.28 kidney brush border membrane Oryctolagus cuniculus
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.28 -60°C, intestinal, renal and amniotic fluid enzyme retain full activity for at least 6 months Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Oryctolagus cuniculus D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 ?
-
 Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 55
-
 1 h, stable Oryctolagus cuniculus
3.2.1.28 60
-
 t1/2: 30 min, in presence of 1% v/v Triton t1/2 is 12 min Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 6
-
-
 Oryctolagus cuniculus