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Literature summary extracted from
- Purification and characterization of acid trehalase from the yeast suc2 mutant [published erratum appears in J Biol Chem 1988 Nov 5; 263(31):16512] (1988), J. Biol. Chem., 263, 8537-8543.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | Hg2+ | 10 mM HgCl2, 30% loss of activity | Saccharomyces cerevisiae |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 4.7 | - |
trehalose | - |
Saccharomyces cerevisiae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.28 | vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 41000 | - |
x * 41000, endoglycosidase H digestion before SDS-PAGE | Saccharomyces cerevisiae |
| 3.2.1.28 | 218000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Saccharomyces cerevisiae | - |
suc2 mutant | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | glycoprotein | 86% carbohydrate | Saccharomyces cerevisiae |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | - |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 99 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | trehalose + H2O | - |
Saccharomyces cerevisiae | D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | ? | x * 41000, endoglycosidase H digestion before SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 4.5 | - |
- |
Saccharomyces cerevisiae |
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