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Literature summary extracted from

  • Arakawa, H.; Clark, W.G.; Psenak, M.; Coscia, C.J.
    Purification and characterization of dihydrobenzophenanthridine oxidase from elicited Sanguinaria canadensis cell cultures (1992), Arch. Biochem. Biophys., 299, 1-7.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.12 1,4-DL-dithiothreitol
-
 Sanguinaria canadensis
1.5.3.12 dicoumarin
-
 Sanguinaria canadensis
1.5.3.12 diethyldithiocarbamate
-
 Sanguinaria canadensis
1.5.3.12 dithiothreitol
-
 Sanguinaria canadensis
1.5.3.12 glutathione
-
 Sanguinaria canadensis
1.5.3.12 iodoacetamide
-
 Sanguinaria canadensis
1.5.3.12 KCN
-
 Sanguinaria canadensis
1.5.3.12 mercaptoethanol
-
 Sanguinaria canadensis
1.5.3.12 Sodium azide
-
 Sanguinaria canadensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.12 0.006
-
 Dihydromacarpine dihydrosanguinarine Sanguinaria canadensis
1.5.3.12 0.012
-
 Dihydrochelerythrine
-
 Sanguinaria canadensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.3.12 copper might be a copper metalloenzyme Sanguinaria canadensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.12 additional information Sanguinaria canadensis one of the enzymes that catalyzes a later step in the biogenesis of benzo(c)phenanthridine alkaloids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.12 Sanguinaria canadensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.12
-
 Sanguinaria canadensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.12 cell culture elicited cells Sanguinaria canadensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.12 additional information
-
-
 Sanguinaria canadensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.12 dihydrochelerythrine + O2
-
 Sanguinaria canadensis chelerythrine + H2O2
-
 ?
1.5.3.12 Dihydromacarpine + O2
-
 Sanguinaria canadensis Macarpine + H2O2
-
 r
1.5.3.12 dihydrosanguinarine + O2
-
 Sanguinaria canadensis sanguinarine + H2O2
-
 ?
1.5.3.12 additional information no reaction with: dihydronorsanguinarine, ajmalicine Sanguinaria canadensis ?
-
 ?
1.5.3.12 additional information one of the enzymes that catalyzes a later step in the biogenesis of benzo(c)phenanthridine alkaloids Sanguinaria canadensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.12 ? a major component of MW 77000 and two minor components with MW between 59000 and 67000 are detected by SDS-PAGE Sanguinaria canadensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.12 7
-
-
 Sanguinaria canadensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.12 6 8.5 about 50% of maximal activity at pH 6.0 and 8.5 Sanguinaria canadensis