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Literature summary extracted from

  • De la Vega, M.G.; Cejudo, F.J.; Paneque, A.
    Purification and properties of an extracellular invertase from Azotobacter chroococcum (1991), Enzyme Microb. Technol., 13, 267-271.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 aniline
-
 Azotobacter chroococcum
3.2.1.26 Ba2+
-
 Azotobacter chroococcum
3.2.1.26 Ca2+
-
 Azotobacter chroococcum
3.2.1.26 Cd2+
-
 Azotobacter chroococcum
3.2.1.26 Cu2+
-
 Azotobacter chroococcum
3.2.1.26 Ni2+
-
 Azotobacter chroococcum
3.2.1.26 pyridoxal 5'-phosphate
-
 Azotobacter chroococcum
3.2.1.26 pyridoxamine
-
 Azotobacter chroococcum
3.2.1.26 Zn2+
-
 Azotobacter chroococcum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 extracellular
-
 Azotobacter chroococcum
-
-
3.2.1.26 intracellular
-
 Azotobacter vinelandii 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 57000
-
 gel filtration Azotobacter chroococcum
3.2.1.26 59000
-
 1 * 59000, SDS-PAGE Azotobacter chroococcum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Azotobacter chroococcum
-
 ATCC 4412
-
3.2.1.26 Azotobacter vinelandii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.26 glycoprotein enzyme can be freed of its carbohydrate component without loss of its catalytic activity Azotobacter chroococcum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26
-
 Azotobacter chroococcum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 0.02
-
-
 Azotobacter chroococcum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 sucrose + H2O
-
 Azotobacter vinelandii D-fructose + D-glucose
-
 ?
3.2.1.26 sucrose + H2O
-
 Azotobacter chroococcum D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 monomer 1 * 59000, SDS-PAGE Azotobacter chroococcum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 45
-
-
 Azotobacter chroococcum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 100
-
 sucrose
-
 Azotobacter chroococcum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 6.5
-
-
 Azotobacter chroococcum