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Literature summary extracted from

  • Lee, H.S.; Sturm, A.
    Purification and characterization of neutral and alkaline invertase from carrot (1996), Plant Physiol., 112, 1513-1522.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 ATP inhibition of neutral invertase, no inhibition of alkaline invertase Daucus carota
3.2.1.26 Ca2+ CaCl2; inhibition of alkaline invertase no inhibition of neutral invertase Daucus carota
3.2.1.26 CTP inhibition of neutral invertase, no inhibition of alkaline invertase Daucus carota
3.2.1.26 fructose competitive inhibitor of neutral and alkaline enzyme Daucus carota
3.2.1.26 glucose non-competitive inhibitor of neutral and alkaline invertase Daucus carota
3.2.1.26 GTP inhibition of neutral invertase, no inhibition of alkaline invertase Daucus carota
3.2.1.26 Hg2+ no inhibition of neutral and alkaline invertase Daucus carota
3.2.1.26 Mg2+ MgCl2, inhibition of alkaline invertase no inhibition of neutral invertase Daucus carota
3.2.1.26 Mn2+ MnCl2, inhibition of alkaline invertase no inhibition of neutral invertase Daucus carota
3.2.1.26 UTP inhibition of neutral invertase, no inhibition of alkaline invertase Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 14.3
-
sucrose pH 6.8, neutral invertase Daucus carota
3.2.1.26 15.1
-
sucrose pH 8.0, alkaline invertase Daucus carota
3.2.1.26 18.7
-
sucrose pH 7.5, neutral invertase Daucus carota
3.2.1.26 20
-
sucrose neutral and alkaline invertase Daucus carota
3.2.1.26 20.7
-
raffinose pH 7.5, alkaline invertase Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 57000
-
8 * 57000, neutral invertase, SDS-PAGE Daucus carota
3.2.1.26 126000
-
4 * 126000, alkaline invertase, SDS-PAGE Daucus carota
3.2.1.26 456000
-
neutral invertase, gel filtration Daucus carota
3.2.1.26 504000
-
alkaline invertase, gel filtration Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Daucus carota
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26
-
Daucus carota

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 additional information
-
-
Daucus carota

Storage Stability

EC Number Storage Stability Organism
3.2.1.26 4°C, 50% glycerol, stable for several days Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 raffinose + H2O no activity with alkaline invertase, hydrolysis with neutral invertase Daucus carota fructose + melibiose
-
?
3.2.1.26 stachyose + H2O no activity with alkaline invertase, hydrolysis with neutral invertase Daucus carota ?
-
?
3.2.1.26 sucrose + H2O alkaline invertase is highly specific for sucrose, neutral invertase also hydrolyzes raffinose and stachyose Daucus carota D-fructose + D-glucose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 octamer 8 * 57000, neutral invertase, SDS-PAGE Daucus carota
3.2.1.26 tetramer 4 * 126000, alkaline invertase, SDS-PAGE Daucus carota

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 37 40
-
Daucus carota

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 20
-
rapid loss of activity above Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 6.8
-
neutral invertase Daucus carota
3.2.1.26 8
-
alkaline invertase Daucus carota

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 4.8 8 pH 4.8: about 35% of maximal activity, pH 8.0: about 60% of maximal activity, neutral invertase Daucus carota
3.2.1.26 7 9 pH 7.0: about 50% of maximal actiovity, pH 9.0: about 70% of maximal activity, alkaline invertase Daucus carota

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.26 7 8 alkaline and neutral invertase, stable in presence of protease inhibitors Daucus carota