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Literature summary extracted from

  • Cuezzo de Gines, S.; Maldonado, M.C.; Font de Valdez, G.
    Purification and characterization of invertase from Lactobacillus reuteri CRL 1100 (2000), Curr. Microbiol., 40, 181-184.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.26 EDTA 10 mM, 47% increase in activity Limosilactobacillus reuteri
3.2.1.26 iodoacetamide enhances activity Limosilactobacillus reuteri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 2-mercaptoethanol
-
 Limosilactobacillus reuteri
3.2.1.26 Ca2+
-
 Limosilactobacillus reuteri
3.2.1.26 Cd2+ 1 mM, 66% inhibition Limosilactobacillus reuteri
3.2.1.26 Cu2+ 1 mM, 90% inhibition Limosilactobacillus reuteri
3.2.1.26 dithiothreitol
-
 Limosilactobacillus reuteri
3.2.1.26 Hg2+ 1 mM, 67% inhibition Limosilactobacillus reuteri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 6.66
-
 sucrose
-
 Limosilactobacillus reuteri

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Limosilactobacillus reuteri
-
 CRL 1100
-
3.2.1.26 Limosilactobacillus reuteri CRL 1100
-
 CRL 1100
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.26 glycoprotein contains 48% carbohydrate Limosilactobacillus reuteri

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26
-
 Limosilactobacillus reuteri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 additional information
-
-
 Limosilactobacillus reuteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 raffinose + H2O
-
 Limosilactobacillus reuteri fructose + melibiose
-
 ?
3.2.1.26 raffinose + H2O
-
 Limosilactobacillus reuteri CRL 1100 fructose + melibiose
-
 ?
3.2.1.26 stachyose + H2O
-
 Limosilactobacillus reuteri ?
-
 ?
3.2.1.26 stachyose + H2O
-
 Limosilactobacillus reuteri CRL 1100 ?
-
 ?
3.2.1.26 sucrose + H2O
-
 Limosilactobacillus reuteri D-fructose + D-glucose
-
 ?
3.2.1.26 sucrose + H2O
-
 Limosilactobacillus reuteri CRL 1100 D-fructose + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 37
-
-
 Limosilactobacillus reuteri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.26 20 45 20°C: about 80% of maximal activity, 45°C: about 70% of maximal activity Limosilactobacillus reuteri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 45
-
 pH 4.5-7.0, stable below Limosilactobacillus reuteri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 6
-
-
 Limosilactobacillus reuteri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 4.5 7 pH 4.5: about 35% of maximal activity, pH 7.0: about 45% of maximal activity Limosilactobacillus reuteri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.26 4.5 7 45°C, stable Limosilactobacillus reuteri
3.2.1.26 5 6 stable Limosilactobacillus reuteri